Coming soon
Practical
Paper Code:
ZOL-115
Credits:
4
Contact Hours:
120.00
Max. Marks:
100.00
Objective:
Course objectives:
This course will enable the students to –
1. Differentiate laboratory specimens based on their external features and appearance of various phyla.
2. Construct knowledge about various fixation and staining techniques using different biological specimens.
3. Appraise their knowledge about different mitotic stages by preparing temporary slides of the onion root tip and visualizing the stages by their key features.
4. Postulate various qualitative and quantitative biochemical techniques to differentiate between different biomolecules based on their physical and chemical properties.
5. Analyze the effect of temperature on the enzymatic activity and gain hands-on expertise in the separation of various solutes using chromatographic techniques.
Course Outcomes (COs):
Animal Diversity I & II
● Study of museum specimens and slides:
● Protozoa:Paramecium(WM), Binary fission, Conjugation, Entamoeba, Trypanosoma, Plasmodium, Monocystis), Opalina, Vorticella, Balantidium (Rectal Ciliate, Nyctotherus(Rectal Ciliate), Euglena, Giardia, Elphidium
● Porifera:Sycon L.S & T.S, Sponge spicules, Spongin fibres
● Leucosolenia, Euplectella, Spongilla.
● Coelenterata: Hydra, Obelia(polyp), Aurelia, Sea anemone, Gorgonia, Physalia, Vellela, Alcyonium, Millepora, Pennatula
● Platyhelminthes:Planaria, Fasciola,Taenia-- Scolex, Gravid proglottid, Fasicola hepatica and its Larval Forms
● Aschelminthes:Ascaris(Male & Female), Dracunculus, Wuchereria, Hook Worm
● Any one animal from Minor Phyla: Ctenophora, Rotifera, Onychophora
Cell Biology
● Fixation and staining:
● Fixatives:- Carnoys, Bouins, formalin
● Stains:- Acetocarmine,Acetorcein, Eosin, Haematoxylin
● Double & Single staining :
● Staining of Euglena,Hydra,Obelia,Foraminiferousshell,Spongespicules,Spongegemmules,Spongin fibre
● Mitosis- Onion root tip (Temporary Slide preparation)
● Meiosis- (Study of Permanent slides)
Biochemistry
● Identification of lipids, proteins & Carbohydrates in the given sample
● Identification of different kinds of mono, di & polysaccharides in the given sample
● Quantitative analysis of cholesterol and glycogen using colorimeter
● Separation of Dyes by circular paper chromatography method
● Analysis of enzymatic activity
● Liver Catalase
Essential Readings:
• Vertebrates Comparative anatomy, function, evolution, K.V. Kardong, Tata-McGraw Hill,New Delhi.
• Practical Zoology, vertebrates, Lal, S.S. Rastogi Publications, Meerut
• Practical Zoology, vertebrates,Kohli,K.S.,Ansari, A.S. Ramesh Book Depot, Jaipur.
• Introductory practical Biochemistry, Sawhney S.K., Singh R. 2009. 7th edition, Narosa publishing house Pvt. Ltd.
• Wilson,K. and Walker, J. Principles and techniques of practical biochemistry, Cambridge press.
• Jordon, E.L., Verma P.S. Invertebrate Zoology, S Chand and Co.
• Laboratory manual in Invertebrate & Experimental Zoology, Jacob VyasRamesh Book Depot, New Delhi.
References:
SUGGESTED READINGS:
● Zubay G. Biochemistry, William C. Brown Pub.
● Standard methods of biochemical analysis.
● Thimmaiah, S.R. KalyaniPublishers,Noida
e-RESOURCES:
• Experimental Biochemistry- NPTEL- https://nptel.ac.in/courses/104105102
• Biochemistry virtual lab- Amrita- https://vlab.amrita.edu/?sub=3&brch=64
• Amrita Virtual Labs- http://biotech01.vlabs.ac.in/List%20of%20experiments.html
Academic Year:
