PRACTICAL

Paper Code: 
ZOL 215
Credits: 
4
Contact Hours: 
120.00
Max. Marks: 
100.00
Objective: 

 

 

Course objectives:

This course will enable the students to -

 

  1. Analyze invertebrate laboratory specimens based on their external features and appearance
  2. Implement molecular biology techniques including gel electrophoresis and chromatography and their applications in the research field.
  3. Differentiate wild and mutant strains of Drosophila.
  4. Perform qualitative and quantitative biochemical techniques to differentiate between different biomolecules based on their physical and chemical properties.
  5. Analyze different modes of central tendency, standard deviation and variance.

 

Course Outcomes (COs):

 

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment

Strategies

Paper Code

Paper Title

ZOL-215

Practical

 

The students will:

CO 41: Identify different laboratory specimens based on their external features and appearance and their categorical relation with various phylum.

CO 42: Get hands-on experience of various molecular biology techniques including gel electrophoresis and chromatography and would learn about their applications in the research field.

CO 43: Enhance their knowledge about wild and mutant strains of Drosophila and their important structures.

CO 44: Apply qualitative and quantitative biochemical techniques to differentiate between different biomolecules based on their physical and chemical properties.

CO 45: Perform calculations of different modes of central tendency, standard deviation and will be able to calculate variance.

The approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching

 

Learning activities for the students:

Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Field practical, demonstration by virtual software.

 

Class test

Semester end examinations,

Quiz,

Assignments,

Presentation

Surprise test

Open book test

 

Biochemistry II

Colorimetric/Spectrophotometric estimation of:

  • Carbohydrate-Glucose
  • Preparation of Phosphate buffer
  • Isolation of protein (casein) from milk
  • Study of temperature effect on enzyme action: Salivary amylase

 

Animal Diversity III

Study of museum specimens and slides:

 

Phylum  Annelida-Nereis, Heteronereis-parapodia, Earthworm, Leech

Dissection (Demonstration): Earthworm- Anatomy, Digestive, Nervous System

Permanent mount:Septalnephridia, Ovary,Nerve ring.

          

Phylum Arthropoda: Daphnia, CyCOps, Scorpion, King Crab, Crab, Eupagurus, Balanus, Millipede, Termite, Centipede, Praying-mantis, Peripatus

Dissection: (Demonstration): Prawn-General anatomy, Appendages, Nervous & Digestive system

Permanent mount: Hastate plate, Statocyst

 

Phylum-Mollusca:Chiton, Unio, Dentalium, Nautilus, Loligo, Octopus, Sepia

 

Phylum Echinodermata: Echinus, Starfish, Cucumaria, Antedon

 

Larval forms- Miracidium, Sporocyst, Redia, Cercaria,Nauplius,Zoea,Mysis,cypris,Megalopa, Glochidium larva.

Molecular Biology:

  •  DNA isolation and separation by Agarose gel method
  • Vertical and horizontal electrophoresis unit (demonstration only)
  • Paper chromatographic separation of amino acids

 

Genetics &Biostatistics

  • Study of Drosophila life cycle & idea about its culture
  • Identification of Male & Female Drosophila
  • Identification of Wild & mutant (Yellow body, Ebony, Vestigial wings, White eye, Apterus) of  Drosophila

Study of permanent prepared slide - Sex comb, Salivary gland chromosomes

 

 

Academic Year: 
2021-2022
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