Practical

Paper Code: 
ZOL 515
Credits: 
4
Contact Hours: 
120.00
Max. Marks: 
100.00
Objective: 
This course will enable the students to -
 
  • Understand principle and working of various instruments in biotechnology laboratory
  • Prepare culture media for microbes
  • Culture bacteria from various samples like soil, food, water etc
  • Perform ELISA and Ouchterlony double diffusion
  • Analyze blood groups

Course Outcomes (COs):

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment 

Strategies

Paper Code

Paper Title

ZOL-515

Practical

Students will 

CO 97: Design experiments based on principle and working of various instruments in biotechnology laboratory

CO 98: Formulate culture media for microbes

CO 99: Examine bacterial isolation from various samples like soil, food, water etc.

CO100: Investigate ELISA and Ouchterlony double diffusion

CO 101: Analyze blood groups

The approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, 

Learning activities for the students:

Self- learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Practical skills and laboratory reports,

Class test 

Semester end examinations

Viva voce

 

Biotechnology 

Principle and working of equipments used in biotechnology laboratory(SDS-PAGE apparatus, horizontal DNA Electrophoresis chamber, Laminar Airflow, Autoclave, Incubator, pH meter, Homogenizer, Ascending and Descending Chromatographic chamber, Thin Layer Chromatographic Apparatus, Microtome)

Microbiology:

  • Preparation and use culture media for microbes

  • Study of microbes in food material(curd, milk, rotten fruits and vegetables)

  • Educational tour to any Microbiology lab (Food processing factory, Distillery, Dairy etc.) Candidates will have to submit a report on it.

  • Culturing of Bacteria from soil/water samples.

  • Quality testing of milk

  • Disc diffusion assay

  • Spread plate and Streak Plate

 

Immunology:

Experiments on Antigen and Antibody reaction.

  • Blood typing

  • ELISA

  • Ouchterlony double diffusion

Academic Year: 
2021-2022
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