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PHYSIOLOGY AND BIOCHEMISTRY PRACTICAL
Course objectives:
This course will enable the students to -
1. Conduct hematological experiments and correlate their values.
2. Develop skills to perform qualitative and quantitative estimation of biomolecules.
3. Learn detailed knowledge of histology of various mammalian tissues.
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Course |
Learning outcomes (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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Course Code |
Course Title |
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25CZOL 302 |
PHYSIOLOGY AND BIOCHEMISTRY PRACTICAL |
CO 31: Design and conduct experiments related to hematology and various qualitative and quantitative tests to identify carbohydrates, proteins and lipids. CO 32: Perceive detailed knowledge about histology of different tissues of mammals. CO 33: Get hands-on training on the experiments related to protein, enzymes and separation of amino acids. CO 34: Demonstrate effective writing and creative skills through practical records. CO 35: Develop skills to defend the expert panel during viva voce exam. CO36:Contribute effectively in course- specific interaction |
The approach in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching Learning activities for the students: Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Field practical |
Assessment Strategies Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation. |
1. R.B.C., W.B.C. count and Haematocrit value
2. Detection of human blood group
3. Determination of bleeding time and clotting time
4. Estimation of Hemoglobin content in the blood sample
5. Study of permanent histological sections of mammalian stomach, pancreas
6. Study of permanent slides of ileum, liver, lung, kidney, bone
7. Qualitative tests of functional groups in carbohydrates, proteins and lipids.
8. Paper chromatography of amino acids
9. Action of salivary amylase under optimum conditions.
10. Enzymatic activity in liver (catalase)
SCHEME FOR PRACTICAL EXAMINATION:
Scheme for CA End Practical Examination
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Exercise 1 |
Exercise II |
File |
Viva |
Attendance |
Total |
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5 |
5 |
10 |
5 |
5 |
30 |
Scheme for Semester End Practical Examination
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Exercise I |
Exercise II |
Exercise III |
Exercise IV |
Spotting |
File |
Viva |
Total |
|
8 |
8 |
8 |
8 |
6x3=18 |
10 |
10 |
70 |
ESSENTIAL READINGS:
● Introductory practical Biochemistry, Sawhney S.K., Singh R. 2009. 7th edition, Narosa publishing house Pvt. Ltd.
● Wilson,K. and Walker, J. Principles and techniques of practical biochemistry, Cambridge press.
● Physiology and biochemistry, Das A.,(2006). 4th edition, Books & Allied (P) Ltd.
SUGGESTED READINGS:
● Zubay G. Biochemistry, William C. Brown Pub.
● Standard methods of biochemical analysis.Thimmaiah, S.R. KalyaniPublishers,Noida ∙ Biology of Animals. C.P. Hickmann, L.S. Roberts and A. Larson. McGraw Hill Company, New York.
● Practical Zoology, Jacob, D., Bhatia,A.L., Kohli,K.S., Vyas,D.K. Ramesh Book Depot, New Delhi.
e-CONTENT:
● Experimental Biochemistry- NPTEL- https://nptel.ac.in/courses/104105102 ∙
● Biochemistry virtual lab-Amrita-https://vlab.amrita.edu/?s ub=3& brch=64 ∙ https://vlab.amrita.edu/?sub=3& brch=64&sim=1090&cnt=2
● Amrita Virtual Labs- http://biotech01.vlabs.ac.in/Lis t%20of%20experiments.html ∙ https://www.youtube.com/watch?v=DN-meIV- G3c
● https://www.youtube.com/watch?v=F 6qj648_Mdk
● https://www.youtube.com/watch?v=1xHA 3EncGmA
● https://www.youtube.com/watch?v=mWAEIvu1mV8
