MOLECULAR BIOLOGY AND BIOTECHNOLOGY

Paper Code: 
25ZOL223
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
This course will enable the students to –
1. Develop an understanding of the life processes at the subcellular and molecular levels.
2. Differentiate and evaluate important aspects of DNA and molecular genetics including gene cloning, sequencing and gene mapping in addition to the powerful techniques that revolutionized the pharmaceutical, health and agricultural industries.
 
Course Outcomes: 

Course

Learning outcomes (at course level)

Learning and teaching strategies

Assessm ent Strategi es

Course Code

Course Title

 

 

 

 

 

 

 

 

 

 

25ZOL

223

 

 

 

 

 

 

 

 

 

 

Molecular Biology And Biotechnology

(Theory)

CO49: Develop an understanding of concepts and mechanisms of replication

CO50: Appraise a fundamental understanding of transcription and post transcriptional modifications. CO51:  Develop an understanding of the translation, post translational modifications and recombination process.

CO52: Evaluate practical and theoretical aspects of different molecular mapping techniques.

CO53: Evaluate the principle and techniques in recombinant DNA technology which holds application in biomedical   and                    genomic science, agriculture etc.

CO54:                          Contribute effectively in course- specific interaction

Approach in teaching: Group Discussion, Reading assignments, PowerPoint presentations Demonstrati on, Guest lecture Learning activities for the students: Effective questions, Simulation, Seminar presentation and Self- learning assignments, Field practical

Class test Semester end examinat ions

Quiz Assignm ents Presentati on Model or

chart preparatio n

 

9.00
Unit I: 
DNA Replication
● Prokaryotic & eukaryotic DNA replication
● Mechanics of DNA replication
● Enzymes and accessory proteins involved in DNA replication
● DNA Repair mechanism 
 
11.00
Unit II: 
Transcription
● Prokaryotic and Eukaryotic transcription
● RNA polymerases
● General and specific transcription factors Post transcriptional modification in RNA
● 5’ cap formation
● 3’ end processing and polyadenylation
● Splicing
● Nuclear export of mRNA
● mRNA editing
 
12.00
Unit III: 
Translation
● Genetic code
● Prokaryotic and eukaryotic translation
● Translational machinery
● Mechanisms of Initiation, Elongation and termination
● Regulation of Translation
● Co and post translational modifications of protein Recombination ● Homologous recombination: Holiday junction,
● Site FLP/ FRT and Cre/lox recombination
● Rec A and other Recombinases
● Gene targeting, Gene disruption
14.00
Unit IV: 
Molecular mapping of Genome
● Genetic and physical maps
● Southern and fluorescence in situ hybridization for genome analysis
● Molecular markers in genome analysis: RFLP, RAPD and AFLP analysis
14.00
Unit V: 
Principle and technique of genetic engineering
● Cutting and joining of DNA molecules
● Cloning strategies, gene library and cDNA
● Application of recombinant DNA technology
❖ Recombination selection and screening
❖ Nucleic acid probes and their application Transgenic animals and knockouts
 
Essential Readings: 
● Molecular Biology of the gene; Watson J.D.; Benzamin /Cummings.
● Molecular Biology of the Cell (2nd Edition) B. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts, and J.D. Watson, Garland publishing. Inc., New York, 1994
Molecular Cell biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore, Scientific AmericanBooks,USA,1994.
● The cell. A Molecular Approach, (4th edition), G H Cooper and R E Hausman, ASM Press,2007.
● Cell & Molecular Biology, (8th edition), E D P Robertis & E M F Robertis, Lippincott Williams & Wilkins,2005
● The cell, (5th edition), R C Swanson and P C Webster , Prentice hall of India Pvt. Ltd., 1990
● Cell and Molecular Biology, (3rd edition), P Sheeler and D E Bianchi, John Wiley & Sons, Inc,1987
Cell and Molecular Biology: Concepts and Experiments, (4th edition), G Karp, JohnWiley & sons, Inc.,2005
● Gene VII, Indian eds. Lewin, B. Oxford university press,Bombay
● Cell Biology by C.B. Powar; Himalaya Publishing House.
● Cell and Molecular Biology; Phillip Sheeler Donald E. Bianchi; John Wiley and Sons.
References: 
SUGGESTED READING:
● The Cell: A Molecular Approach. Cooper, G. Houseman G.R. SinaeurAssoc, New York
● Becker, W.M., Kleinsmith, L.J., Hardin. J. and Bertoni, G. P. (2009).The World of the Cell.VII Edition. Pearson Benjamin Cummings Publishing, San
● Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts,
● Peter Walter: Molecular Biology of the Cell, IV Edition.
● Cooper G. M. and Robert E. Hausman R. E. The Cell: A Molecular Approach, V Edition, ASM Press and Sinauer Associates.
● De Robertis, E.D.P. and De Robertis, E.M.F. (2006).Cell and Molecular Biology.VIII Edition. Lippincott Williams and Wilkins, Philadelphia.
● Karp, G. (2010) Cell and Molecular Biology: Concepts and Experiments. VI Edition.John Wiley and Sons.Inc.
● Lewin B. (2008). Gene XI, Jones and Bartlett
● McLennan A., Bates A., Turner, P. and White M. (2015). Molecular Biology IV Edition.GS, Taylor and Francis Group, New York and London.
● Molecular Biology of the gene; Watson J.D.; Benzamin/ Cummings.
● Molecular Cell biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore, Scientific American Books,USA,1994.
● Cell & Molecular Biology, (8th edition), E D P Roberties& E M F Roberties, Lippincott Williams & Wilkins, 2005
● Cell and Molecular Biology, (3rd edition), P Sheeler and D E Bianchi, John Wiley & Sons, Inc, 1987
 
e RESOURCES:
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JOURNALS:
The Journal of Cell and Molecular Biology Cellular and Molecular Biology
● Cellular and Molecular Life Science 
 
Academic Year: 
2025-2026
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