Molecular Biology and Biotechnology

Paper Code: 
ZOL 223
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course objectives:

This course will enable the students to –

  1. Develop an understanding of the life processes at the subcellular and molecular levels.
  2. Differentiate and evaluate important aspects of DNA and molecular genetics including gene cloning, sequencing and gene mapping in addition to the powerful techniques that revolutionized the pharmaceutical, health and agricultural industries.

 

Course Outcomes (COs):

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

ZOL 223

 

Molecular Biology And Biotechnology

The students will –

CO43: Develop an understanding of concepts, mechanisms and evolutionary significance and relevance of molecular biology in the current scenario.

CO44: Differentiate and evaluate in recombinant DNA technology which holds application in biomedical and genomic science, agriculture etc.

CO45: Appraise a fundamental understanding of Molecular Biology will help in career building in all these fields.

CO46: Apply their knowledge in problem solving and future course of their career development in higher education and research.

CO47: Evaluate practical and theoretical aspects of different molecular techniques.

 

Approach in teaching:

 

Group Discussion, Reading assignments, PowerPoint presentations

Demonstration, Guest lecture

 

Learning activities for the students:

Effective questions, Simulation, Seminar presentation and Self-learning assignments, Field practical

 

Class test

Semester end examinations

Quiz

Assignments

Presentation

Model or chart preparation

 

9.00
Unit I: 
UNIT I
DNA Replication
● Prokaryotic & eukaryotic DNA replication
● Mechanics of DNA replication
● Enzymes and accessory proteins involved in DNA replication
● DNA Repair mechanism
 
11.00
Unit II: 
UNIT II
Transcription
● Prokaryotic and Eukaryotic transcription
● RNApolymerases
● General and specific transcription factors
Post transcriptional modification in RNA
● 5’ cap formation
● 3’ end processing and polyadenylation
● Splicing
● Nuclear export ofmRNA
● mRNAediting
 
12.00
Unit III: 
UNIT III

Translation

  • Genetic code
  • Prokaryotic and eukaryotic translation
  • Translationalmachinery
  • Mechanisms of Initiation, Elongation and termination
  • Regulation ofTranslation
  • Co and post translational modifications of protein Recombination
  • Homologous recombination:Holiday junction,
  • Site FLP/ FRT and Cre/lox recombination
  • Rec A and otherRecombinases
  • Gene targeting, Gene disruption

 

14.00
Unit IV: 
UNIT IV

Molecular mapping of Genome

  • Genetic and physical maps
  • Southern and fluorescence in situ hybridization for genome analysis
  • Molecular markers in genome analysis: RFLP,RAPDand AFLPanalysis

 

14.00
Unit V: 
UNIT V

Principle and technique of genetic engineering

  • Cutting and joining of DNAmolecules
  • Cloning strategies, gene library andcDNA
  • Application of recombinant DNAtechnology
    • Recombination selection and screening
    • Nucleic acid probes and their application Transgenic animals and knockouts

 

Essential Readings: 
● Molecular Biology of the gene; Watson J.D.; Benzamin /Cummings.
● Molecular Biology of the Cell (2nd Edition) B. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts, and J.D. Watson, Garland publishing. Inc., New York, 1994Molecular Cell biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore, Scientific AmericanBooks,USA,1994.
● The cell. A Molecular Approach, (4th edition), G H Cooper and R E Hausman, ASM Press,2007.
● Cell & Molecular Biology, (8th edition), E D P Robertis & E M F Robertis, Lippincott Williams & Wilkins,2005
● The cell, (5th edition), R C Swanson and P C Webster , Prentice hall of India Pvt. Ltd., 1990
● Cell and Molecular Biology, (3rd edition), P Sheeler and D E Bianchi, John Wiley & Sons, Inc,1987
● Cell and Molecular Biology: Concepts and Experiments, (4th edition), G Karp, John Wiley & sons, Inc.,2005
● Gene VII, Indian eds. Lewin, B. Oxford university press,Bombay
● Cell Biology by C.B. Powar; Himalaya Publishing House. 
● Cell and Molecular Biology; Phillip Sheeler Donald E. Bianchi; John Wiley and Sons.
 
References: 
SUGGESTED READING:
● The Cell: A Molecular Approach. Cooper, G. Houseman G.R. SinaeurAssoc, New York
● Becker, W.M., Kleinsmith, L.J., Hardin. J. and Bertoni, G. P. (2009).The World of the Cell.VII  Edition. Pearson Benjamin Cummings Publishing, San  
● Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts,  
● Peter Walter: Molecular Biology of the Cell, IV Edition. 
● Cooper G. M. and Robert E. Hausman R. E. The Cell: A Molecular Approach, V Edition, ASM  Press and Sinauer Associates. 
● De Robertis, E.D.P. and De Robertis, E.M.F. (2006).Cell and Molecular Biology.VIII Edition.  Lippincott Williams and Wilkins, Philadelphia. 
● Karp, G. (2010) Cell and Molecular Biology: Concepts and Experiments. VI Edition.John Wiley  and Sons.Inc. 
● Lewin B. (2008). Gene XI, Jones and Bartlett 
● McLennan A., Bates A., Turner, P. and White M. (2015). Molecular Biology IV Edition.GS,  Taylor and Francis Group, New York and London. 
● Molecular Biology of the gene; Watson J.D.; Benzamin/ Cummings. 
● Molecular Cell biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore, Scientific American  Books,USA,1994. 
● Cell & Molecular Biology, (8th edition), E D P Roberties& E M F Roberties, Lippincott Williams  & Wilkins, 2005 
● Cell and Molecular Biology, (3rd edition), P Sheeler and D E Bianchi, John Wiley & Sons, Inc,  1987 
e RESOURCES:
JOURNALS:
The Journal of Cell and Molecular Biology
Cellular and Molecular Biology 
Cellular and Molecular Life Sciences
 
Academic Year: 
2022-2023
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