Molecular Biology and Biotechnology

Paper Code: 
ZOL 223
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
9.00
Unit I: 
UNIT I

DNA Replication

  • Prokaryotic & eukaryotic DNA replication
  • Mechanics of DNA replication
  • Enzymes and accessory proteins involved in DNA replication

DNA Repair mechanism

11.00
Unit II: 
UNIT II

Transcription

  • Prokaryotic and Eukaryotic  transcription
  • RNA polymerases
  • General and specific transcription factors

Post transcriptional modification in RNA

  • 5’ cap formation
  • Transcription termination
  • 3’ end processing and polyadenylation
  • Splicing
  • Nuclear export of mRNA
  • mRNA stability

 

12.00
Unit III: 
UNIT III

Translation

  • Genetic code
  • Prokaryotic and eukaryotic translation
  • Translational machinery
  • Mechanisms of  Initiation, Elongation and termination
  • Regulation of Translation
  • Co and post translational modifications of protein

Recombination and repair

Holiday junction, Gene targeting, Gene disruption

FLP/ FRT and Cre/lox recombination

Rec A and other recombinases

 

9.00
Unit IV: 
UNIT IV

Molecular mapping of Genome

  • Genetic and physical maps
  • Southern and fluorescence in situ hybridization for genome analysis
  • Molecular markers in genome analysis: RFLP,RAPD and AFLP analysis

 

14.00
Unit V: 
UNIT V

Principle and technique of genetic engineering

  • Cutting and joining of DNA molecules

·         Cloning strategies, gene library and cDNA

·         Application of recombinant DNA technology

o   Recombination selection and screening

o   Nucleic acid probes and their application

Transgenic animals and knock outs    

Essential Readings: 
  • Molecular Biology of the gene; Watson J.D.; Benzamin/ Cummings.
  • Molecular Biology of the Cell (2nd Edition) B. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts, and J.D. Watson, Garland publishing. Inc., New York, 1994.
  • Molecular Cell biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore, Scientific American Books,USA,1994.
  • Cell & Molecular Biology, (8th edition), E D P Roberties & E M F Roberties,  Lippincott Williams & Wilkins, 2005
  • The cell, (5th edition), R C Swanson and P C Webster , Prentice hall of India Pvt. Ltd., 1990
  • Cell and Molecular Biology, (3rd edition), P Sheeler and D E Bianchi, John Wiley & Sons, Inc, 1987
  • Cell and Molecular Biology: Concepts and Experiments, (4th edition), G  Karp, John Wiley & sons, Inc., 2005
  • The cell. A Molecular Approach, (4th edition), G H Cooper and R E Hausman, ASM Press, 2007
  • Gene VII, Indian eds. Lewin, B. Oxford university press, Bombay

 

 

Academic Year: 
2015-2016
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