Coming soon
MOLECULAR BIOLOGY AND BIOTECHNOLOGY
Course objectives:
This course will enable the students to -
- The course provides an insight into the life processes at the subcellular and molecular levels.
- Other important aspects include DNA and molecular genetics including gene cloning, sequencing and gene mapping in addition to the powerful techniques that revolutionized the pharmaceutical, health and agricultural industries.
Course Outcomes (COs):
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Course |
Learning outcomes (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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Paper Code |
Paper Title |
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ZOL 223
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Molecular Biology And Biotechnology |
Students will be able to: CO31: Develop an understanding of concepts, mechanisms and evolutionary significance and relevance of molecular biology in the current scenario.
CO32: Get well versed in recombinant DNA technology which holds application in biomedical and genomic science, agriculture, environment management, etc. Therefore, a fundamental understanding of Molecular Biology will help in career building in all these fields.
CO33: Apply their knowledge in problem solving and future course of their career development in higher education and research. |
Approach in teaching: Group Discussion, Reading assignments, PowerPoint presentations Demonstration, Guest lecture
Learning activities for the students: Effective questions, Simulation, Seminar presentation and Self learning assignments, Field practical
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Class test Semester end examinations Quiz Assignments Presentation Model or chart preparation |
DNA Replication
- Prokaryotic & eukaryotic DNA replication
- Mechanics of DNA replication
- Enzymes and accessory proteins involved in DNA replication
- DNA Repair mechanism
Transcription
- Prokaryotic and Eukaryotic transcription
- RNA polymerases
- General and specific transcription factors
Post transcriptional modification in RNA
- 5’ cap formation
- 3’ end processing and polyadenylation
- Splicing
- Nuclear export of mRNA
- mRNA editing
Translation
- Genetic code
- Prokaryotic and eukaryotic translation
- Translational machinery
- Mechanisms of Initiation, Elongation and termination
- Regulation of Translation
- Co and post translational modifications of protein
Recombination
- Homologous recombination:Holiday junction,
- Site FLP/ FRT and Cre/lox recombination
- Rec A and other Recombinases
- Gene targeting, Gene disruption
Molecular mapping of Genome
- Genetic and physical maps
- Southern and fluorescence in situ hybridization for genome analysis
- Molecular markers in genome analysis: RFLP,RAPD and AFLP analysis
Principle and technique of genetic engineering
- Cutting and joining of DNA molecules
- Cloning strategies, gene library and cDNA
- Application of recombinant DNA technology
- Recombination selection and screening
- Nucleic acid probes and their application
Transgenic animals and knock outs
Essential Readings
Molecular Biology of the gene; Watson J.D.; Benzamin/ Cummings.
Molecular Biology of the Cell (2nd Edition) B. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts, and J.D. Watson, Garland publishing. Inc., New York, 1994.
Molecular Cell biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore, Scientific American Books,USA,1994.
The cell. A Molecular Approach, (4th edition), G H Cooper and R E Hausman, ASM Press, 2007.
Suggested Readings
Cell & Molecular Biology, (8th edition), E D P Robertis & E M F Robertis, Lippincott Williams & Wilkins, 2005
The cell, (5th edition), R C Swanson and P C Webster , Prentice hall of India Pvt. Ltd., 1990
Cell and Molecular Biology, (3rd edition), P Sheeler and D E Bianchi, John Wiley & Sons, Inc, 1987
Cell and Molecular Biology: Concepts and Experiments, (4th edition), G Karp, John Wiley & sons, Inc., 2005
Gene VII, Indian eds. Lewin, B. Oxford university press, Bombay
