Molecular Biology and Biotechnology

Paper Code: 
ZOL-223
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

The course Objectives to provide students with a basic understanding of:

  1. Roles of various biomolecules in the cell
  2. How the DNA in a genome is organized, replicated, and repaired
  3. How genetic information in the DNA is selectively expressed as functional proteins.
  4. How genetic information transferred in the forms of codons and post translational modifications.
  5. Molecular mapping of Genome
  6. Principle and technique of genetic engineering

Learning outcomes- By the end of the course, students should be able to:

  1. Describe the Roles of various biomolecules in the cell
  2. Describe the process and importance of DNA replication.
  3. Describe the process of Transcription and translation in the cell.
  4. Describe Molecular mapping of Genome
  5. Describe Principle and technique of genetic engineering
9.00
Unit I: 
UNIT I

DNA Replication

  • Prokaryotic & eukaryotic DNA replication
  • Mechanics of DNA replication
  • Enzymes and accessory proteins involved in DNA replication

DNA Repair mechanism

11.00
Unit II: 
UNIT II

Transcription

  • Prokaryotic and Eukaryotic  transcription
  • RNA polymerases
  • General and specific transcription factors

Post transcriptional modification in RNA

  • 5’ cap formation
  • 3’ end processing and polyadenylation
  • Splicing
  • Nuclear export of mRNA

mRNA editing

12.00
Unit III: 
UNIT III

Translation

  • Genetic code
  • Prokaryotic and eukaryotic translation
  • Translational machinery
  • Mechanisms of  Initiation, Elongation and termination
  • Regulation of Translation
  • Co and post translational modifications of protein

Recombination

  • Homologous recombination:Holiday junction,
  • Site FLP/ FRT and Cre/lox recombination
  • Rec A and other Recombinases

Gene targeting, Gene disruption

14.00
Unit IV: 
UNIT IV

Molecular mapping of Genome

  • Genetic and physical maps
  • Southern and fluorescence in situ hybridization for genome analysis

Molecular markers in genome analysis: RFLP,RAPD and AFLP analysis

14.00
Unit V: 
UNIT V

Principle and technique of genetic engineering

  • Cutting and joining of DNA molecules
  • Cloning strategies, gene library and cDNA
  • Application of recombinant DNA technology
    1. Recombination selection and screening
    2. Nucleic acid probes and their application

Transgenic animals and knock outs   

Essential Readings: 
  • Molecular Biology of the gene; Watson J.D.; Benzamin/ Cummings.
  • Molecular Biology of the Cell (2nd Edition) B. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts, and J.D. Watson, Garland publishing. Inc., New York, 1994.
  • Molecular Cell biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore, Scientific American Books,USA,1994.
  • The cell. A Molecular Approach, (4th edition), G H Cooper and R E Hausman, ASM Press, 2007.
References: 
  • Molecular Biology of the gene; Watson J.D.; Benzamin/ Cummings.
  • Molecular Biology of the Cell (2nd Edition) B. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts, and J.D. Watson, Garland publishing. Inc., New York, 1994.
  • Molecular Cell biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore, Scientific American Books,USA,1994.
  • Cell & Molecular Biology, (8th edition), E D P Roberties& E M F Roberties,  Lippincott Williams & Wilkins, 2005
  • The cell, (5th edition), R C Swanson and P C Webster , Prentice hall of India Pvt. Ltd., 1990
  • Cell and Molecular Biology, (3rd edition), P Sheeler and D E Bianchi, John Wiley & Sons, Inc, 1987
  • Cell and Molecular Biology: Concepts and Experiments, (4th edition), G  Karp, John Wiley & sons, Inc., 2005
  • Gene VII, Indian eds. Lewin, B. Oxford university press, Bombay
Academic Year: 
2019-2020
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