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MOLECULAR BIOLOGY
Paper Code:
24DZOL812
Credits:
4
Contact Hours:
60.00
Max. Marks:
100.00
Objective:
Course objectives:
This course will enable the students to –
1. Develop an understanding of the life processes at the subcellular and molecular levels. 2. Differentiate and evaluate important aspects of DNA and molecular genetics including gene cloning, sequencing and gene mapping in addition to the powerful techniques that revolutionized the pharmaceutical, health and agricultural industries.
Course Outcomes:
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Course |
Learning outcomes (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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Course Code |
Course Title |
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24DZOL 802 |
Molecular Biology THEORY
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CO:229 Develop an understanding of concepts and mechanisms of replication CO:230 Appraise a Fundamental understanding of transcription and post transcriptional modifications. CO:231 Develop an understanding of the translational and post translational process. CO:232 Evaluate practical and theoretical aspects of different molecular mapping techniques. CO 233 Compare and contrast between different DNA recombination and repair techniques. CO:234 Contribute effectively in course-specific interaction |
Approach in teaching: Reading assignments, PowerPoint presentations Guest lecture Learning activities for the students: Simulation, Seminar presentation and Self-learning assignments,
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Class test Semester end examinations Quiz Assignments Presentation Model or chart preparation |
12.00
Unit I:
DNA Replication
Prokaryotic & eukaryotic DNA replication
Mechanics of DNA replication
Enzymes and accessory proteins involved in DNA replication
12.00
Unit II:
Transcription
Prokaryotic and Eukaryotic transcription
RNA polymerases
General and specific transcription factors
Post transcriptional modification in RNA
5’ cap formation
3’ end processing and polyadenylation
Splicing
Nuclear export of mRNA
mRNA editing
12.00
Unit III:
Translation
Genetic code
Prokaryotic and eukaryotic translation
Translational machinery
Mechanisms of Initiation, Elongation and termination
Regulation of Translation
Co and post translational modifications of protein
12.00
Unit IV:
Molecular mapping
Molecular mapping of Genome
Genetic and physical maps
Southern and fluorescence in situ hybridization for genome analysis
Molecular markers in genome analysis: RFLP, RAPD and AFLP analysis
12.00
Unit V:
Recombination and Repair
Homologous recombination: Holiday junction,
Site FLP/ FRT and Cre/lox recombination
Rec A and other Recombinases
DNA Repair mechanism : Mismatch repair, Excision repair, Direct repair, Recombination repair,
Double stranded break repair, SOS repair
Essential Readings:
ESSENTIAL READING:
● Molecular Biology of the gene; Watson J.D.; Benzamin /Cummings.
● Molecular Biology of the Cell (2nd Edition) B. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts, and J.D. Watson, Garland publishing. Inc., New York, 1994
● Molecular Cell biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore, Scientific AmericanBooks,USA,1994.
● The cell. A Molecular Approach, (4th edition), G H Cooper and R E Hausman, ASM Press,2007.
● Cell & Molecular Biology, (8th edition), E D P Robertis & E M F Robertis, Lippincott Williams & Wilkins,2005
● The cell, (5th edition), R C Swanson and P C Webster , Prentice hall of India Pvt. Ltd., 1990
● Cell and Molecular Biology, (3rd edition), P Sheeler and D E Bianchi, John Wiley & Sons, Inc,1987
● Cell and Molecular Biology: Concepts and Experiments, (4th edition), G Karp, John Wiley & sons, Inc.,2005
● Gene VII, Indian eds. Lewin, B. Oxford university press,Bombay
● Cell Biology by C.B. Powar; Himalaya Publishing House.
● Cell and Molecular Biology; Phillip Sheeler Donald E. Bianchi; John Wiley and Sons.
SUGGESTED READING:
● The Cell: A Molecular Approach. Cooper, G. Houseman G.R. SinaeurAssoc, New York
● Becker, W.M., Kleinsmith, L.J., Hardin. J. and Bertoni, G. P. (2009).The World of the Cell.VII Edition. Pearson Benjamin Cummings Publishing, San
● Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts,
● Peter Walter: Molecular Biology of the Cell, IV Edition.
● Cooper G. M. and Robert E. Hausman R. E. The Cell: A Molecular Approach, V Edition, ASM Press and Sinauer Associates.
● De Robertis, E.D.P. and De Robertis, E.M.F. (2006).Cell and Molecular Biology.VIII Edition. Lippincott Williams and Wilkins, Philadelphia.
● Karp, G. (2010) Cell and Molecular Biology: Concepts and Experiments. VI Edition.John Wiley and Sons.Inc.
● Lewin B. (2008). Gene XI, Jones and Bartlett
● McLennan A., Bates A., Turner, P. and White M. (2015). Molecular Biology IV Edition.GS, Taylor and Francis Group, New York and London.
● Molecular Biology of the gene; Watson J.D.; Benzamin/ Cummings.
● Molecular Cell biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore, Scientific American Books,USA,1994.
● Cell & Molecular Biology, (8th edition), E D P Roberties& E M F Roberties, Lippincott Williams & Wilkins, 2005
● Cell and Molecular Biology, (3rd edition), P Sheeler and D E Bianchi, John Wiley & Sons, Inc, 1987
e CONTENT:
JOURNALS:
The Journal of Cell and Molecular Biology
Cellular and Molecular Biology
Cellular and Molecular Life Sciences
Academic Year:
