MOLECULAR BIOLOGY

Paper Code: 
CZOL-513T
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course 

Learning outcomes 

(at course level)

Learning and  teaching strategies

Assessment  Strategies 

Paper  

Code

Paper Title

CZOL-513T 

Molecular Biology

The students will:

CO 1: Relate different terminologies related to chromosomes and their morphology, packaging of a long thread of DNA into the nucleus and the role of associated proteins in this process.

 

CO 2: Appraise the structure and types of Nucleic acids, their characteristic features and occurrence.

 

CO 3: Develop detailed knowledge about the process and importance of DNA replication in both prokaryotes and eukaryotes and protein machinery involved into it.

 

CO 4: Analyze comprehensive knowledge about the process of Transcription, translation along with the mechanisms of post transcriptional and post-translational processes in the cell.

 

The approach in  teaching: 

Interactive Lectures,  Discussion, Tutorials,  Reading assignments,  Demonstration, Team  teaching 

Learning activities for  the students: 

Self-learning  

assignments, Effective  questions, Simulation,  Seminar presentation,  Giving tasks, Chart  preparation

Assessment  Strategies 

Class test,  Semester end  examinations,  Quiz, Solving  problems in  tutorials,  

Assignments,  Presentati

 

12.00
Unit I: 
Unit I

Nucleic Acids

Salient features of DNA and RNA

Watson and Crick model of DNA

Semi-conservative, bidirectional and semi-discontinuous replication, 

DNA Replication: DNA Replication in prokaryotes and eukaryotes, 

mechanism of DNA replication, RNA priming, replication of telomeres

 
12.00
Unit II: 
Unit II

Transcription

 

RNA polymerase and transcription Unit, 

mechanism of transcription in prokaryotes and eukaryotes

transcription factors

 
12.00
Unit III: 
Unit III

Translation 

 

Genetic code, Degeneracy of the genetic code and Wobble Hypothesis; 

Process of protein synthesis in prokaryotes: 

Ribosome structure and assembly in prokaryotes, fidelity of protein synthesis, aminoacyl tRNA synthetases and charging of tRNA; 

Proteins involved in initiation, elongation and termination of polypeptide chain; Inhibitors of protein synthesis

Difference between prokaryotic and eukaryotic translation

 
12.00
Unit IV: 
Unit IV

Post Transcriptional Modifications and Processing of Eukaryotic RNA 

Split genes: concept of introns and exons, splicing mechanism, alternative splicing, exon shuffling, 

RNA editing, Processing of tRNA

 

12.00
Unit V: 
Unit V

Gene Regulation: Transcription regulation in prokaryotes: Principles of transcriptional regulation with examples from lac operon and trp operon

Transcription regulation in eukaryotes: Activators, repressors, enhancers, silencer elements; Gene silencing, Genetic imprinting

DNA Repair Mechanisms : Pyrimidine dimerization and mismatch repair

Regulatory RNAs : Ribo-switches, RNA interference, miRNA, siRNA

 
Essential Readings: 

ESSENTIAL READING:

 

● Cell Biology by C.B. Powar; Himalaya Publishing House.

● Cell and Molecular Biology; Phillip Sheeler Donald E. Bianchi; John Wiley and Sons.

 

SUGGESTED READING:

 

● The Cell: A Molecular Approach. Cooper, G. Houseman G.R. SinaeurAssoc, New York

● Becker, W.M., Kleinsmith, L.J., Hardin. J. and Bertoni, G. P. (2009). The World of the

Cell.VII Edition. Pearson Benjamin Cummings Publishing, San

● Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts,

● Peter Walter: Molecular Biology of the Cell, IV Edition.

● Cooper G. M. and Robert E. Hausman R. E. The Cell: A Molecular Approach, V Edition,

ASM Press and Sinauer Associates.

● De Robertis, E.D.P. and De Robertis, E.M.F. (2006).Cell and Molecular Biology.VIII

Edition. Lippincott Williams and Wilkins, Philadelphia.

● Karp, G. (2010) Cell and Molecular Biology: Concepts and Experiments. VI Edition.John

Wiley and Sons.Inc.

● Lewin B. (2008). Gene XI, Jones and Bartlett

● McLennan A., Bates A., Turner, P. and White M. (2015). Molecular Biology IV

Edition.GS, Taylor and Francis Group, New York and London.

● Molecular Biology of the gene; Watson J.D.; Benzamin/ Cummings.

● Molecular Cell biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore, Scientific

American Books,USA,1994.

● Cell & Molecular Biology, (8th edition), E D P Roberties& E M F Roberties, Lippincott

Williams & Wilkins, 2005

● Cell and Molecular Biology, (3rd edition), P Sheeler and D E Bianchi, John Wiley & Sons,

Inc, 1987

 

e-RESOURCES:

• https://epgp.inflibnet.ac.in/Home/ViewSubject?catid=2rAs1Puvga4LW93zMe83...

• https://nptel.ac.in/courses/104103121

• https://www.swayamprabha.gov.in/index.php/program/archive/9

 

JOURNALS:

• The Journal of Cell and Molecular Biology

• Cellular and Molecular Biology

• Cellular and Molecular Life Sciences

 
Academic Year: 
2023-2024
  • Printer-friendly version
  • PDF version