Molecular Biology

Paper Code: 
ZOL 213
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
Objective: 

Course objectives:

 

This course will enable the students to –

 

  1. Analyze terminologies related to chromosomes and their morphology, packaging of long threads of DNA into the nucleus and the role of associated proteins in this process.
  2. Discriminate the structure and types of Nucleic acids, their characteristic features and occurrence.
  3. Appraise the process and importance of DNA replication in both prokaryotes and eukaryotes and protein machinery involved into it.
  4. Analyze the process of Transcription, Translation along with the mechanisms of post transcriptional and post translational processes in the cell.

 

Course Outcomes (COs):

 

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment

Strategies

Paper Code

Paper Title

ZOL 213

Molecular Biology

The students will:

CO 32: Relate different terminologies related to chromosomes and their morphology, packaging of a long thread of DNA into the nucleus and the role of associated proteins in this process.

CO 33: Appraise the structure and types of Nucleic acids, their characteristic features and occurrence.

CO 34: Develop detailed knowledge about the process and importance of DNA replication in both prokaryotes and eukaryotes and protein machinery involved into it.

CO 35: Analyze comprehensive knowledge about the process of Transcription, translation along with the mechanisms of post transcriptional and post-translational processes in the cell.

The approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Guest lectures

Learning activities for the students:

Self learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Regular activities like quiz, surprise test, explanation of given task

 

Class test

Semester end examinations,

Quiz,

Assignments,

Presentation

Surprise test

Open book test

 

 

9.00
Unit I: 
UNIT I
  • Chromosomes: Morphology, Chromonema, Chromomeres, Telomeres, Primary & secondary constrictions, Chromatids
  • Chromosome type- Polytene&Lampbrush
  • Chromosomal Organization: Euchromatin, Heterochromatin , Nucleosome concept

 

7.00
Unit II: 
UNIT II

Nucleosides,

  • Nucleotides
  • DNA
  • DNA structure(A, B, Z type)
  • RNA structure & types( mRNA, rRNA, tRNA)

 

10.00
Unit III: 
UNIT III
  • Replication of prokaryotes (Semi-conservative mechanism: Experiments of Messelson& Stahl)
  • Prokaryotic Polymerases, Topoisomerases, Single stranded binding protein, Replication forks(Unidirectional & Bidirectional), Leading & lagging strand, RNA primers & Okazaki fragments
  • Brief Idea of Eukaryotic Replication( Ori sites and Enzymes involved)

 

10.00
Unit IV: 
UNIT IV
  • Prokaryotic Transcription
  • RNA Polymerases
  • Mechanism of transcription
  • Brief idea about transcription factors in eukaryotic transcription    
  • Post Transcriptional modification in Eukaryotes
  • Capping
  • Polyadenylation
  • Splicing

 

9.00
Unit V: 
UNIT V
  • Genetic Code
  • Prokaryotic translation
  • Translational Machinery
  • Translational Initiation, Elongation and termination                                                                                   

 

Essential Readings: 
  • Cell Biology by C.B. Powar; Himalaya Publishing House.
  • Cell and Molecular Biology; Phillip Sheeler   Donald E. Bianchi; John Wiley and Sons.
  • The Cell: A Molecular Approach. Cooper, G. Houseman G.R. SinaeurAssoc, New York
  • Becker, W.M., Kleinsmith, L.J., Hardin. J. and Bertoni, G. P. (2009).The World of the Cell.VII Edition. Pearson Benjamin Cummings Publishing, San
  • Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts,
  • Peter Walter: Molecular Biology of the Cell, IV Edition.
  • Cooper G. M. and Robert E. Hausman R. E. The Cell: A Molecular Approach, V Edition, ASM Press and Sinauer Associates.
  • De Robertis, E.D.P. and De Robertis, E.M.F. (2006).Cell and Molecular Biology.VIII Edition. Lippincott Williams and Wilkins, Philadelphia.
  • Karp, G. (2010) Cell and Molecular Biology: Concepts and Experiments. VI Edition.John Wiley and Sons.Inc.
  • Lewin B. (2008). Gene XI, Jones and Bartlett
  • McLennan A., Bates A., Turner, P. and White M. (2015).Molecular Biology IV Edition.GS, Taylor and Francis Group, New York and London.
  • Molecular Biology of the gene; Watson J.D.; Benzamin/ Cummings.
  • Molecular Cell biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore, Scientific American Books,USA,1994.
  • Cell & Molecular Biology, (8th edition), E D P Roberties& E M F Roberties,  Lippincott Williams & Wilkins, 2005
  • Cell and Molecular Biology, (3rd edition), P Sheeler and D E Bianchi, John Wiley & Sons, Inc, 1987
  • The cell. A Molecular Approach, (4th edition), G H Cooper and R E Hausman, ASM Press, 2007

 

Academic Year: 
2021-2022
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