Microbiology

Paper Code: 
ZOL 513
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
Objective: 

Course objectives:

This course will enable the students to -

  1. Describe the structure of the bacterial cell
  2. To gain knowledge about genetic material and reproductive methods in bacteria.
  3. To understand the nutritional requirements and culture of the microbes.
  4. To describe the epidemiology of infectious agents including how infectious diseases are transmitted.
  5. To explain interventions employed to prevent infectious diseases including infection control measures and vaccines.

Course Outcomes (COs):

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment

Strategies

Paper Code

Paper Title

ZOL-513

Microbiology

The student will be able to

CO 90: Understand  morphology of bacterial cell, its genetic material and modes of reproduction

CO 91: Gain knowledge about nutritional requirements of bacteria and culture the microbes.

CO 92: Describe the epidemiology of infectious agents including how infectious diseases are transmitted.

CO 93: The student will be able to explain interventions employed to prevent infectious diseases including infection control measure and vaccines

 

The approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, PowerPoint presentations, Explanation by videos

 

Learning activities for the students:

Self learningassignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Field practical

 

Class test

Semester end examinations,

Quiz,

Assignments,

Presentation

Surprise test

Open book test

 

9.00
Unit I: 
UNIT I

Brief introduction to the history of Microbiology

  • Work of Anton Van Leeuwenhoek
  • Work of Louis Pasteur, John Tyndall, Robert Koch & Jenner

Prokaryota(Bacteria)

  • Size, shape & pattern of arrangement
  • Structural Organization—Slime layer(Capsule) Cell envelope, Cytoplasmic membrane(Inner membrane), Cell wall(Outer membrane) of gram-ve and  gram +ve bacteria, Mesosomes; Cytoplasmic organization, Cell projections- Flagella and pili

 

8.00
Unit II: 
UNIT II
  • Genetic material of bacteria: Chromosomes, Plasmids, Replication of bacterial DNA
  • Reproduction in Bacteria: Asexual reproduction: Binary fission, budding, Endospore formation& cyst formation Sexual reproduction: Conjugation
  • Transduction, Bacterial recombination and transformation

 

10.00
Unit III: 
UNIT III

Microbial nutrition culture of Bacteria

  • Carbon & energy source
  • Nitrogen & minerals
  • Oxygen
  • Organic growth factors
  • Microbial growth
  • Environmental factors: Temperature, Hydrogen ion concentration, Osmotic pressure

 

10.00
Unit IV: 
UNIT IV

Bacteria of medical importance:

Gram +ve:
    Cocci : Staphylococci, Streptococci
    Bacilli: Diptheria, Tetanus

Gram –ve:
    Cocci: Gonnorrhea, Meningitis
    Bacilli: Diarrhoea

The causative agents, Transmission, Pathogenecity, Laboratory diagnosis, Treatment & Prevention of:
    Mycobacteria: Tuberculosis
    Hepatitis (with emphasis on B type)

 

8.00
Unit V: 
UNIT V

Cyanobacteria (Structural organization and significance),

 Virus: General Characteristics, structure, classification on the basis of morphology and genetic material

 Lytic & Lysogenic cycles

 yeast- industrial application

Essential Readings: 
  • Jacob D.Introductory Microbiology,Ramesh Book Depot,Jaipur.
  • P.D. Sharma, Microbiology,Rastogi and Company, Merrut.
  • David Freifelder-Molecular Biology, Narosa Publishing House, New Delhi.
  • Salle,S.J. Fundamental Principles of Bacteriology,Tata Mc Graw Hill Pub. Co., New Delhi.

Suggested Readings

  • Microbial:T.Stuart Walker,W.B.Saunders Company
  • Microbiological Application,Alfred E.Brown,McGraw.Hill
  • Practical Biochemistry:Principles and Techniques; Keith Wilson& John Walker;Cambridge University Press.
  • Lechiman, W.Microbiology, Glenced Pub1. Co. New York
  • Nestler, R.L. Microbiology, W.S. Saunders, Philadelphia (Student edition:Holt-Saunders International Edition, Tokyo).
  • Stonier, T.I. Nouderroff, and Adeloverg, E.A. General Microbiology.

 

Academic Year: