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Microbiology
Paper Code:
ZOL- 513
Credits:
3
Contact Hours:
45.00
Max. Marks:
100.00
Objective:
Course outcomes:
This course will enable the students to -
1. Critique structure of the bacterial cell
2. Assess genetic material and reproductive methods in bacteria.
3. Understand the nutritional requirements and culture of the microbes.
4. Examine the epidemiology of infectious agents including how infectious diseases are transmitted.
5. Conjecture interventions employed to prevent infectious diseases including infection control measures and vaccines.
Course Outcomes (COs):
9.00
Unit I:
UNIT I:
● Brief introduction to the history of Microbiology
● Work of Anton Van Leeuwenhoek
● Work of Louis Pasteur, John Tyndall, Robert Koch & Jenner
● Prokaryota(Bacteria)
● Size, shape & pattern of arrangement
●Structural Organization—Slime layer(Capsule) Cell envelope, Cytoplasmic membrane(Inner membrane), Cell wall(Outer membrane) of gram-ve and gram +vebacteria, Mesosomes; Cytoplasmic organization, Cell projections- Flagella and pili
8.00
Unit II:
UNIT II:
● Genetic material of bacteria: Chromosomes, Plasmids, Replication of bacterial DNA
● Reproduction in Bacteria: Asexual reproduction: Binary fission, budding, Endospore formation& cyst formation Sexual reproduction: Conjugation
● Transduction, Bacterial recombination and transformation
10.00
Unit III:
UNIT III:
●Microbial nutrition
Nutritional types
Nutritional Requirement (Carbon, Oxygen, Nitrogen, Phosphorus &Sulphar)
Organic growth factors
● Environmental factors: Temperature, Hydrogen ion concentration, Osmotic pressure
● Microbial growth
10.00
Unit IV:
UNIT IV:
● Bacteria of medical importance:
Gram +ve:
● Cocci : Staphylococci, Streptococci
● Bacilli: Diptheria, Tetanus
Gram –ve:
● Cocci: Gonnorrhea, Meningitis
● Bacilli: Diarrhoea
● The causative agents, Transmission, Pathogenecity, Laboratory diagnosis, Treatment &Prevention of:
● Mycobacteria: Tuberculosis
● Hepatitis (with emphasis on B type)
8.00
Unit V:
UNIT V:
● Cyanobacteria (Structural organization and significance),
● Virus: General Characteristics, structure, classification on the basis of morphology and genetic material
● Lytic & Lysogenic cycles
● yeast- industrial application
Essential Readings:
● P.D. Sharma, Microbiology,Rastogi and Company, Meerut.
● Microbiological Application,AlfredE.Brown, McGraw.Hill
● Microbiology by Totora, Pearson Education.
References:
SUGGESTED READINGS:
● Microbial:T.StuartWalker,W.B.Saunders Company
● Microbiological Application,AlfredE.Brown, McGraw.Hill
● Practical Biochemistry:Principles and Techniques; Keith Wilson& John Walker;CambridgeUniversity Press.
● Lechiman, W.Microbiology, Glenced Pub1. Co.New York
● Nestler, R.L. Microbiology, W.S. Saunders, Philadelphia (Student edition:Holt-Saunders International Edition, Tokyo).
● Salle,S.J. Fundamental Principles of Bacteriology,TataMcGraw Hill Pub. Co., New Delhi. ● Stonier, T.I. Nouderroff, and Adeloverg, E.A. General Microbiology.
● Jacob D.IntroductoryMicrobiology,Ramesh Book Depot,Jaipur.
● Cruick shank et al. Medical Microbiology.The English Language Book Society,London.
David Freifelder-Molecular Biology, Narosa Publishing House, New Delhi
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