HUMAN GENETICS (Practical)

Paper Code: 
24ZOL424D
Objective: 
Course objectives:
This course will enable the students to -
1. Develop an understanding about Isolation of genomic DNA from blood and tissues
2. Understand and perform how to separate and culture of lymphocytes
3. Appraise the detailed knowledge about Mendelian traits, DNA sequencing and Dermatoglyphics
 
Course Outcomes: 

Course

Learning outcomes (at course level)

Learning and teaching strategies

Assessme nt Strategies

Course Code

Course Title

 

 

 

 

 

 

 

 

 

 

24ZOL

424D

 

 

 

 

 

 

 

 

 

Human genetics II (Practical)

CO205. Develop an understanding about

Isolation of genomic DNA from blood and tissues

CO206. Understand and perform how to separate and culture of lymphocytes

CO207. Appraise the detailed knowledge about Mendelian traits, DNA sequencing and Dermatoglyphics and Analyse gene & genotype frequencies by Hardy Weinberg’s Law

CO208. Interpret the amino acid sequence data with nucleotide sequence data (BLAST and FASTA)

CO209. Reflect on personal learning experiences and experimental outcomes during viva voce assessments

CO210. Contribute effectively in course-specific interaction

Approach        in teaching: Practical, Reading assignments, Demonstration

Learning activities for the students: Practical knowledge,

Effective questions, Seminar presentation

Class      test Semester end examination s Viva voce

 

● Isolation of Genomic DNA from Chicken blood or mouse tissues
● Hardy-Weinberg Genetic equilibrium: Study of gene & genotype; frequencies. (PTC Tasters & non tasters)
● Mendelian Traits : Straight hair, Curly hair, Widow’s peak, Dimpled Cheeks, Mid digital hair, Hitchhiker's thumb, Clasping of hands, and Hypertrichosis
● Phylogeny based on Amino acid sequences
● Correlation of amino acid sequence data with nucleotide sequence data (BLAST and FASTA)
● Separation and short term culture of lymphocytes
● Dermatoglyphics : Recording of print of fingertips and palm. Classify ridges on the Finger tips arch, loop, and whorl. Palm print – area demark as hypothenar, the nar & inter digital read. Record presence or absence of Simian crease.
 
Essential Readings: 
● Nelson et al, Lehninger’s Principles of Biochemistry, 4th Edition, McMillan, 2006.
● Alberts et al, Molecular Biology of The Cell, 2nd Edition , Garland 2007.
● Lewin, Genes IX, 9th Edition , Jones & Bartlett, 2007.
● Pastemak, An Introduction to Molecular Human Genetics, 2nd Edition, Fritzgarald, 2005..
● Lewis, Human Genetics, 7th Edition, WCB & McGraw, 2007..
● Strachen and Read, Human Molecular Genetics, 3rd Edition, Garland Sci. Publishing, 2004.
● Structural Chemistry and Molecular Biology Alexander Rich and Norman Davidson
● The Chemical Foundations of Molecular :James D. Watson
● Biology of the Gene (2nd ed.)
● Molecular Biology of the Cell ; Lewis and Watson
● Genes VI : Benjamin Lewin
● Human Cytogenetic Techniques and Clinical Applications Hema Purandare and Arnit Chakravarty
● Progress in Nucleic Acid Research and Molecular Biology Vol. 9 J. N. Davidson
● Essentials of Human Genetics Bhatnagar,Kothari,Mehta
 
References: 
SUGGESTED READINGS:
● Genes VII :Benjamin Lewin
● Molecular Biology of the Gene (4th ed.) Watson
● Molecular Biology of the Cell : Alberts. Lewis & Watson
● An introduction to genetic Engineering Desmond S. T. Nicoll
● Principles and Practive of Medical Genetics, by Emery, A.E.H and D.L. Rimoin (Eds_ (1990-2nd edition) Churchill Livingstone, Edinburgh.
● Molecular Basis of Inherited Diseases, (6th Edition-1989) by Scriver, C.R. A.L. Beudit, W.S. Sty abnd D. Valle (Eds0 McGrawHill, New York.
● Human Genetics by S.D. Gangane (2nd edition-Reprint 2001), B.L Churchill Livingstone Pvt. Ltd., New Delhi.
● Genetics in Medicine by M.W. Thompson et al, 5th Edition, W.B. Sounders Company, London
● Genetic basis of common diseases by R. A. King et al, Oxford University Press.
 
E-RESOURCES:
● chrome- extension://efaidnbmnnnibpcajpcglclefindmkaj/https://www.researchgate.net/profile/Worku
-Mhiret- 2/publication/339127895_Laboratory_Manual_for_Principles_of_Genetics/links/5e3ee1d82 99bf1cdb918e9da/Laboratory-Manual-for-Principles-of-Genetics.pdf
 
 
E-JOURNALS:
 
Academic Year: 
2024-2025
  • Printer-friendly version
  • PDF version