HUMAN GENETICS I (Practical)

Paper Code: 
25ZOL324D
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
This course will enable the students to –
1. Hands on experience of Chromosomal preparation and karyotyping
2. Learn to Culture Drosophila and identification of normal and mutant flies
3. Experimental knowledge of DNA isolation, purification and quantification
4. Describe about restriction endonucleases and electrophoresis
 
Course Outcomes: 

Course

Learning outcomes (at course level)

Learning and teaching strategies

Assessment Strategies

Course Code

Course Title

25ZOL

324 D

Human

Genetics I

(Practical)

CO133. Perform

Chromosomal

preparation and

karyotyping

CO134.  Learn  to  Culture

Drosophila in laboratory and

identify normal and  mutant

flies

CO135. Isolate, purify  and

quantify    DNA,     use                 of

Restriction

endonucleases and

Electrophoresis.

CO136.     Explore                 EcoRI

digestion of total DNA.

CO137. Demonstrate skills and record the concepts  in the form  of practical file  and

reflect  on  personal learning experiences and experimental outcomes during viva voce.

CO138.                            Contribute

effectively in course-specific

interaction

Approach in

teaching:

Practical,

Demonstration

Learning  activities

for the students:

Practical                knowledge,

Effective questions

Class test

Semester      end

examinations

Viva voce

 

●Identification of normal and mutant flies ( Drosophila melanogaster) Preparation of Drosophila polytene Chromosomes (temporary and permanent preparations)
Drosophila genetic crosses
Chromosome preparation from mouse bone marrow and human blood lymphocytes G banding
Karyotyping
Identification of inactivated X chromosome as Barr body and drumstick
Genomic DNA isolation and purification from the nuclei and quantitation of DNA by spectrophotometer.
EcoRI digestion of total DNA and separation of fragments by agarose gel Electrophoresis

Exercise 1

Exercise II

File

Viva

Attendance

Total

5

5

10

5

5

30

Scheme for CA End Practical Examination

Exercise I

Exercise II

Exercise III

Exercise IV

Spotting

File

Viva

Total

8

8

8

8

6x3=18

10

10

70

Scheme for M.Sc Semester End Practical Examination

Essential Readings: 
Nelson et al, Lehninger’s Principles of Biochemistry, 4th Edition, McMillan, 2006.
Alberts et al, Molecular Biology of The Cell, 2nd Edition , Garland 2007.
Lewin, Genes IX, 9th Edition , Jones & Bartlett, 2007.
Pastemak, An Introduction to Molecular Human Genetics, 2nd Edition, Fritzgarald, 2005..
Lewis, Human Genetics, 7th Edition, WCB & McGraw, 2007..
Strachen and Read, Human Molecular Genetics, 3rd Edition, Garland Sci. Publishing, 2004.
Structural Chemistry and Molecular Biology Alexander Rich and Norman Davidson
The Chemical Foundations of Molecular :James D. Watson
Biology of the Gene (2nd ed.)
Molecular Biology of the Cell ; Lewis and Watson
Genes VI : Benjamin Lewin
Human Cytogenetic Techniques and Clinical Applications Hema Purandare and Arnit Chakravarty
Progress in Nucleic Acid Research and Molecular Biology Vol. 9 J. N. Davidson
Essentials of Human Genetics Bhatnagar, Kothari, Mehta
SUGGESTED READINGS
Genes VII :Benjamin Lewin
Molecular Biology of the Gene (4th ed.) Watson
Molecular Biology of the Cell : Alberts. Lewis & Watson
An introduction to genetic Engineering Desmond S. T. Nicoll
Principles and Practice of Medical Genetics, by Emery, A.E.H and D.L. Rimoin (Eds_ (1990-2nd edition) Churchill Livingstone, Edinburgh.
Molecular Basis of Inherited Diseases, (6th Edition-1989) by Scriver, C.R.
A.L. Beudit, W.S. Sty abnd D. Valle (Eds0 McGrawHill, New York.
Human Genetics by S.D. Gangane (2nd edition-Reprint 2001), B.L Churchill Livingstone Pvt. Ltd., New Delhi.
Genetics in Medicine by M.W. Thompson et al, 5th Edition, W.B. Sounders Company, London
Genetic basis of common diseases by R. A. King et al, Oxford University 
Press.
 
References: 
E-RESOURCES:
chrome- extension://efaidnbmnnnibpcajpcglclefindmkaj/https://www.researchgate.net/profile/Worku
-Mhiret- 2/publication/339127895_Laboratory_Manual_for_Principles_of_Genetics/links/5e3ee1d82 99bf1cdb918e9da/Laboratory-Manual-for-Principles-of-Genetics.pdf
 
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