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Human genetics I (Practical)
Paper Code:
ZOL 324 D
Credits:
4
Contact Hours:
60.00
Max. Marks:
100.00
Objective:
Course objectives
This course will enable the students to –
- Acquaint Hands on experience of Chromosomal preparation and karyotyping
- Explore to Culture Drosophila and identification of normal and mutant flies
- Appraise Experimental knowledge of DNA isolation, purification and quantification
- Describe about restriction endonucleases and electrophoresis
Course Outcomes (COs):
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Course |
Learning outcomes (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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Paper Code |
Paper Title |
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ZOL 324 D
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Human genetics I (Practical)
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Students will – CO111: Perform Chromosomal preparation and karyotyping CO112:Assess to Culture Drosophila in laboratory and identify normal and mutant flies CO113: Isolate, purify and quantify DNA, use of Restriction endonucleases and Electrophoresis. CO 114: Explore EcoRI digestion of total DNA.
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Approach in teaching: Practical, Reading assignments, Demonstration
Learning activities for the students: Practical knowledge, Effective questions, Seminar presentation
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Class test Semester end examinations Viva voce
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CONTENTS
● Identification of normal and mutant flies ( Drosophila melanogaster) Preparation of Drosophila polytene Chromosomes (temporary and permanent preparations)
● Drosophila genetic crosses
● Chromosome preparation from mouse bone marrow and human blood lymphocytes G-banding
● Karyotyping
● Identification of inactivated X chromosome as Barr body and drumstick
● Genomic DNA isolation and purification from the nuclei and quantitation of DNA by spectrophotometer.
● EcoRI digestion of total DNA and separation of fragments by agarose gel Electrophoresis
Academic Year:
