CELL BIOLOGY PRACTICAL

Paper Code: 
25CZOL414
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
This course will enable the students to –
1. Construct knowledge about various fixation and staining techniques using different biological specimens.
2. Appraise their knowledge about different mitotic stages by preparing temporary slides of the onion root tip and visualizing the stages by their key features.
 
Course Outcomes: 

Course

Learning outcomes (at course level)

Learning and teaching strategies

Assessment Strategies

Course Code

Course Title

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

25CZOL414

 

 

 

 

 

 

 

 

 

 

 

CELL BIOLOGY

Practical

CO:91 Learn to prepare and observe onion root tip squash slides to identify mitotic stages.

CO:92 Study meiosis stages to understand gamete formation.                                Prepare permanent slides to visualize Barr bodies in human female cells.

CO:93 Gain skills in fixation and staining techniques using various fixatives and stains.

CO:94 Understand double and single staining methods for cellular visualization.

CO:95 Demonstrate skills and record the concepts in the form of practical file and reflect on personal learning experiences                                    and

experimental outcomes during viva voce.

CO:96 Contribute effectively in course-specific interaction

The approach in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching

 

Learning activities for the students: Self- learning

assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Chart Preparation

Assessment Strategies Class test, Semester end

examinations, Quiz, Solving problems in tutorials, Assignments, Presentation.

 

CONTENTS:
1. Preparation of temporary stained squash of onion root tip to study various stages of mitosis
2. Study of permanent slides of various stages of mitosis and meiosis.
3. Preparation of permanent slide to show the presence of Barr body in human female blood cells/cheek cells.
4. Fixation and staining:
● Fixatives:- Carnoys, Bouins, formalin
● Stains:- Acetocarmine,Acetorcein, Eosin, Haematoxylin
5. Double & Single staining
 
Essential Readings: 
ESSENTIAL READINGS:
• Practical Zoology, Vertebrates, Lal, S.S. Rastogi Publications, Meerut
• Practical Zoology, Vertebrates,Kohli,K.S.,Ansari, A.S. Ramesh Book Depot, Jaipur.
• Laboratory manual in Invertebrate & Experimental Zoology, Jacob VyasRamesh Book Depot, New Delhi.
 
SUGGESTED READINGS:
● Thimmaiah, S.R. Kalyani Publishers, Noida
 
References: 
e-RESOURCES:
 
Academic Year: 
2025-2026
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