CELL BIOLOGY PRACTICAL

Paper Code: 
24ZOL502(C)
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
Course objectives:
This course will enable the students to –
1. Construct knowledge about various fixation and staining techniques using different biological specimens.
2. Appraise their knowledge about different mitotic stages by preparing temporary slides of the onion root tip and visualizing the stages by their key features.
 
Course Outcomes: 

Course

Learning outcomes (at course level)

Learning and teaching 

strategies

Assessment

Strategies

 Course Code

Course Title

24DZOL502(C)

 

CELL BIOLOGY

PRACTICAL

 

 

CO 79: Learn to prepare and observe onion root tip squash slides to identify mitotic stages.

CO 80: Study meiosis stages to understand gamete formation.

CO 81: Prepare permanent slides to visualize Barr bodies in human female cells.

CO82: Gain skills in fixation and double and single staining techniques using various fixatives and stains for cellular visualization

CO83:Demonstrate skills and recond the concepts in the form of practical file and reflect on personal learning experiences and experimental outcomes during viva voce.

CO84:Contribute effectively in course-specific interaction

The approach in teaching:

Interactive 

Lectures, 

Discussion, 

Tutorials, 

Reading 

assignments, Demonstration, Team teaching Learning 

activities for 

the students:

Self-learning 

assignments, Effective 

questions, 

Simulation, 

Seminar 

presentation, Giving tasks, 

Chart 

preparation

Assessment 

Strategies

Class test, 

Semester end 

examinations, 

Quiz, Solving 

problems in 

tutorials, 

Assignments, 

Presentation.

 

CONTENT
 
Fixation and staining:
Fixatives:- Carnoys, Bouins, formalin
Stains:- Acetocarmine,Acetorcein, Eosin, Haematoxylin
Double & Single staining :
Staining of Euglena, Hydra, Obelia, Foraminiferous shell, Sponge spicules, Sponge gemmules, Spongin fibre
Mitosis- Onion root tip (Temporary Slide preparation) and study of permanent slides
Meiosis- Study of Permanent slides
 
SCHEME FOR PRACTICAL EXAMINATION:
Scheme for CA  End Practical Examination

Exercise  1

Exercise II

File

Viva

Attendance

Total

 

5

 

 

5

 

10

 

5

 

5

 

30

Scheme for Semester End Practical Examination

Exercise I

Exercise II

Exercise III

Exercise IV

Spotting

File

Viva

Total

8

8

8

8

6x3=18

10

10

70

 

Essential Readings: 
ESSENTIAL READINGS: 
Practical Zoology, Vertebrates, Lal, S.S. Rastogi Publications, Meerut 
Practical Zoology, Vertebrates,Kohli,K.S.,Ansari, A.S. Ramesh Book Depot, Jaipur. 
Laboratory manual in Invertebrate & Experimental Zoology, Jacob VyasRamesh Book  Depot, New Delhi.
 
SUGGESTED READINGS: 
Thimmaiah, S.R. Kalyani Publishers, Noida 
 
e-RESOURCES: 
Academic Year: