CELL BIOLOGY PRACTICAL

Paper Code: 
24CZOL414
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
Course objectives: 
This course will enable the students to –
1. Construct knowledge about various fixation and staining techniques using different biological specimens.
2. Appraise their knowledge about different mitotic stages by preparing temporary slides of the onion root tip and visualizing the stages by their key features.
 
 
Course Outcomes: 

Course

Learning outcomes

(at course level)

Learning and 

teaching strategies

Assessment

Strategies

 Course Code

Course Title

 

 

 

24 CZOL 414

CELL BIOLOGY

Practical

CO:91 Learn to prepare and observe onion root tip squash slides to identify mitotic stages.

CO:92 Study meiosis stages to understand gamete formation. Prepare permanent slides to visualize Barr bodies in human female cells.

CO:93 Gain skills in fixation and staining techniques using various fixatives and stains.

CO:94 Understand double and single staining methods for cellular visualization.

CO:95 Demonstrate skills and record the concepts in the form of  practical file and reflect on personal learning experiences and experimental outcomes during viva voce.

CO:96 Contribute effectively in course-specific interaction

The approach in  teaching:

Interactive Lectures,  Discussion, 

Tutorials, Reading  assignments, 

Demonstration, 

Team teaching

 

Learning activities  for the students: Self-learning 

assignments, 

Effective questions,  Simulation, Seminar  presentation, Giving  tasks, Chart 

preparation

Assessment 

Strategies

Class test, Semester  end examinations,  Quiz, Solving 

problems in 

tutorials, 

Assignments, 

Presentation.

 

CONTENTS: 
1. Preparation of temporary stained squash of onion root tip to study various stages of mitosis 
2. Study of permanent slides of various stages of mitosis and  meiosis. 
3. Preparation of permanent slide to show the presence of Barr body in human female blood cells/cheek cells. 
4. Fixation and staining: 
              ● Fixatives:- Carnoys, Bouins, formalin 
                ● Stains:- Acetocarmine,Acetorcein, Eosin, Haematoxylin 
5. Double & Single staining 
 

SCHEME FOR PRACTICAL EXAMINATION:

Scheme for CA  End Practical Examination

Exercise  1

Exercise II

File

Viva

Attendance

Total

 

5

 

 

5

 

10

 

5

 

5

 

30

 

Scheme for Semester End Practical Examination

Exercise I

Exercise II

Exercise III

Exercise IV

Spotting

File

Viva

Total

8

8

8

8

6x3=18

10

10

70

 

 

ESSENTIAL READINGS: 
• Practical Zoology, Vertebrates, Lal, S.S. Rastogi Publications, Meerut 
• Practical Zoology, Vertebrates,Kohli,K.S.,Ansari, A.S. Ramesh Book Depot, Jaipur. 
• Laboratory manual in Invertebrate & Experimental Zoology, Jacob VyasRamesh Book  Depot, New Delhi.
 
SUGGESTED READINGS: 
● Thimmaiah, S.R. Kalyani Publishers, Noida 
 
e-RESOURCES: 
 
Academic Year: