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CELL BIOLOGY
Paper Code:
25ZOL122
Credits:
4
Contact Hours:
60.00
Max. Marks:
100.00
Objective:
This course will enable the students to –
1. Formulate basic concepts of cellular structure and function covering all the components of prokaryotic and eukaryotic cells, especially macromolecules, membranes, and organelles.
2. Investigate an account of the complex regulatory mechanisms that control cell functions therefore it will help students to understand how these cellular components are used to generate and utilize energy in cells.
Course Outcomes:
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Course |
Learning outcomes (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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Course Code |
Course Title |
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25ZOL 122 |
Cell Biology (Theory) |
CO7:Explore cellular diversity, including prokaryotic and eukaryotic cells, Cell organelles, Role of cytoskeleton. CO8:Expplain the structure of Biomembranes along with transport across membranes CO9:Appraise the detailed knowledge of different pathways related to cell signaling CO10:Investigate role of Cell-cell adhesion and Cell matrix adhesion molecules in various types of cell junctions CO11:Analyze protein sorting and transport CO12:Contribute effectively in course-specific interaction |
Approach in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, PowerPoint presentations Learning activities for the students: Self learning assignments, Effective questions, Simulation, Seminar presentation, |
Class test Semester end examinations, Quiz, Assignments, Presentation Open book test |
9.00
Unit I:
UNIT I
● Cell Theory
● Structure of prokaryotic and eukaryotic cells.
● Cell organelles-ER, Golgi, Mitochondria, Ribosomes, Peroxisomes
● Cytoskeleton: Structure, Function and Dynamics of Microfilaments: Actin and Myosin
Microtubule in mitosis.
- Kinesin and Dynein.
Intermediate Filaments
9.00
Unit II:
Bio membranes
● Phospholipids: main lipid constituent.
● Cytosolic and exoplasmic face of bio membranes.
● Intrinsic and extrinsic proteins.
● Glycolipids.
● Mobility of lipids and integral proteins
● Fluidity of bio membrane.
Transport across cell membranes.
● Passive Transport
● Diffusion & Facilitated Diffusion
● Osmosis and water channels.
● Active Transport- pumps, ABC transporters
● Cotransport by symporters and antiporters
15.00
Unit III:
15 hrs Cell-Cell Signalling.
● Endocrine, paracrine, autocrine signalling.
● Receptor Proteins-Intracellular Receptors.
● Cell surface receptors-G-protein coupled receptors, ion channel receptors, tyrosine kinase linked receptors and receptors with intrinsic enzymatic activity.
● Second messenger system-cAMP, IP3-DAG and MAP kinase pathways.
● Signal-Mediated transport through Nuclear Pore.
● Nuclear Pore Complex.
● Nuclear export signals.
● Nuclear localization signals
12.00
Unit IV:
Cell Junctions: Occluding, Adherence and communicating junctions
Cell- cell adhesion molecules
● Selectin
● Cadherin
● Integrin
● Ig Superfamily
Cell matrix adhesion
● Integrins
● Collagen
Non-collagen components of extracellular matrix
15.00
Unit V:
Protein sorting and transport
● Protein secretion- Regulative and Secretory Pathways
● Targeting proteins to Endoplasmic Reticulum
● Insertion of protein into ER membrane
● Protein folding and processing in ER
● Export of proteins from ER
● Cargo selection, coat protein and vesicle budding
● Post translational modification in Golgi
● Protein sorting and export from Golgi
Essential Readings:
● IWASA, J., MARSHALL, W. F., & KARP, G. (2016). Karp's cell and molecular biology: concepts and experiments.
● COOPER, G. M., & HAUSMAN, R. E. (2009). The cell: a molecular approach. Washington, D.C., ASM Press.
● Nelson et al, Lehninger’s Principles of Biochemistry, 4th Edition, McMillan, 2006.
● Alberts et al, Molecular Biology of The Cell, 2nd Edition, Garland 2007.
● Lewin, Genes IX, 9th Edition , Jones & Bartlett, 2007
● Molecular Biology of the Cell ; Lewis and Watson
● Genes VII : Benjamin Lewin
● Lewis, Human Genetics, 7th Edition, WCB & McGraw, 2007.
References:
● Pastemak, An Introduction to Molecular Human Genetics, 2nd Edition, Fritzgarald, 2005.
● Strachen and Read, Human Molecular Genetics, 3rd Edition, Garland Sci. Publishing, 2004.
● Structural Chemistry and Molecular Biology Alexander Rich and Norman Davidson
● The Chemical Foundations of Molecular :James D. Watson
● Human Cytogenetic Techniques and Clinical Applications HemaPurandare and Arnit Chakravarty
● Progress in Nucleic Acid Research and Molecular Biology Vol. 9 J. N. Davidson
● Essentials of Human Genetics Bhatnagar,Kothari,Mehta
● Principles and Practice of Medical Genetics, by Emery, A.E.H and D.L. Rimoin (Eds (1990-2nd edition) Churchill Livingstone, Edinburgh.
● Molecular Basis of Inherited Diseases, (6th Edition-1989) by Scriver, C.R. A.L. Beudit, W.S. Sty ab nd
D. Valle (Eds0 McGrawHill, New York.
● Human Genetics by S.D. Gangane (2nd edition-Reprint 2001), B.L Churchill Livingstone Pvt. Ltd., New Delhi.
● Genetics in Medicine by M.W. Thompson et al, 5th Edition, W.B. Sounders Company, London
● Genetic basis of common diseases by R. A. King et al, Oxford University Press.
● Mendelian inheritance in Man by Mc. Kusick V.A. (1998), 12th Edition, John Hopsins University Press, Baltimore.
e- RESOURCES:
● Cell biology -NPTEL- https://nptel.ac.in/courses/102103012
● Molecular Biology-NPTEL-https://nptel.ac.in/courses/102104052
e- JOURNAL:
● Molecular and Cellular Biology
BMC Cell and Molecular Biology- ISSN: 2661-8850
Academic Year:
