Cell and molecular Biology I(Practical)

Paper Code: 
ZOL 324 B
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
Course objectives:
This course will enable the students to -
 
Examine working and principle of microscope and oculometer.
Hand on experience of various molecular biology techniques.
Acquaint on various chromosomal techniques.
Discover morphology of different types of cells and tissues.
 
Course Outcomes (COs): 
 

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

ZOL 324B

Cell and Molecular Biology (Practical)

Students will –

CO101: Deduce working and principle of microscope and oculometer.

 

CO102: Evaluate experience of various molecular biology techniques.

 

CO103: Infer various chromosomal techniques.

 

CO104: Conclude morphology of different types of cells and tissues

CO105: Perceive knowledge about Chromosomes in chironomus larva and mammalian chromosomes from bone marrow.

 

Approach in teaching:

Practical, Reading assignments, Demonstration

 

Learning activities for the students:

Practical  knowledge, Effective questions, Seminar presentation

 

Class test

Semester end examinations

Viva voce

 

 

CONTENTS
  • Use of microscopes
  • Use of oculometer: standardization and measurements of cell height, nuclear diameter and tubular diameters
  • Use of ocular Grid-standardization and counting of cells or nuclei in cross sectional epithelium.
  • Determination of the absorption maxima of a solution.
  • Determination of relationship between absorption and various concentration of a solution using colorimeter and spectrophotometer.
  • Preparation of standard curve for protein, lipid, carbohydrate and enzymes.
  • Separation Techniques
  • Separation of proteins and DNA by Agaose gel electrophoresis
  • Separation of proteins on SDS PAGE and PAGE
  • Separation of amino Acids by paper chromatography
  • Separation of amino Acids by TLC
  • Chromosomal Techniques
  • Preparation of salivary gland chromosome from chironomus larva and stained with acetocarmine /aceto orcein and feulgen
  • Preparation of mammalian chromosomes from bone marrow
  • Permanent slides-Types of cells (Squamous, cuboidal, columnar epithelial cells, Nerve Cells, Muscle Cells, Connective tissues of various types), adipose tissues, mitotic and meiotic chromosomes and their different phases, Cancer cells of various types.
 
 
 
 
 
Academic Year: 
2021-2022
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