Cell and Molecular biology II

Paper Code: 
ZOL 424B
Credits: 
4
Contact Hours: 
120.00
Max. Marks: 
100.00
Objective: 

Course Outcomes (COs):

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

ZOL

424B

Cell

and Molecular Biology II

(Practical)

 

Students will :

CO159: Evaluate and perform different biochemical methods, Immunoassays and quantitation of antibodies

CO160: Analyse and prepare histological slides by microtomy and  histochemical techniques

CO161: Design and understand various immunohistochemistry protocols

CO162: Differentiate and evaluate detailed practical knowledge of different biochemical methods

Approach in teaching:

Practical, Reading assignments, Demonstration

 

Learning activities for the students:

Practical  knowledge, Effective questions, Seminar presentation

 

Class test

Semester end examinations

Viva voce

 

 

  • Quantitation of antibodies-
    • PrecipitationTechniques
    • Immunodiffusion diffusion method.
  • Preparation of biological tissues and sectioning for paraffin wax histology by microtomy
  • Histochemistry
    • Carbohydrate- PASmethod
    • Proteins- Ninhydrin Method
    • Nucleic acid- Feulgen Method
    • Localization of acid & alkaline phosphatase Ascorbic Acid
  • Determination of optimum concentration of enzyme for kinetic studies
  • Estimation of acid & alkaline phosphatase by biochemical method
  • Immunoassays-ELISA
  • Permanent slides (for Spotting)- Blood cells, Thymus, Spleen,Liver

 

Academic Year: