Cell and Molecular Biology II

Paper Code: 
ZOL 423 B
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
12.00
Unit I: 
UNIT I

Molecular Immunology.

Humoral and Cell mediated immunity.

  • Innate (nonspecific) immunity.
  • Adaptive (specific) Immunity.

 

Cells and organs of immune system.Hematopoiesis

  • B-lymphocytes, T-lymphocytes, Null cells.
  • Mononuclear cells
  • Granulocytic cells     
  • Mast Cells.
  • Dendritic cells.

Organs of immune system.

Lymphatic system.

  • Primary lymphoid organs
  • Secondary    lymphoid    organs   

Antigens.

  • Immunogenicity versus antigenicity.
  • Factors  influence immnogenicity
  • Contribution   of  Biological   system. (Genotype   of  the recipient    animal,    immunogen    dosage    and    route    of
    administration, adjuvants).
  • Epitopes
  • Haptans.

 

12.00
Unit II: 
UNIT II

Immunoglobulins : Structure and function.

·   Molecular structure of Ig, Light chain and Heavy chain

·     Immunoglobulin domains.

 

·   Immunoglobulin classes

·   Immunoglobulin-mediated     effector    functions                                      

·      Antigenic determinants on immunoglobulin (isotype, allotype and idiotype).

 

Generation of antibody diversity.

·                Multiple germ line V, D and J gene segments.

·   Combinatorial V-J and V-D-J joining.

·   Junctional flexibility.

·   P-addition and N-addition.

·   Association of heavy and light chain.

Class switching among constant region genes.

·   Expression of Ig genes.

·   Regulation of Ig gene transcription.

Antibody genes and antibody engineering

12.00
Unit III: 
UNIT III

Antigen-Antibody Interaction.( in vivo and in vitro)

Major Histocompatibility complex.

  • General organization and inheritance of MHC.
  • Genomic maps of MHC genes.
  • MHC molecules and genes.

 

  • Cellular distribution of MHC molecules.
  • Regulation of MHC expression.
  • MHC and immune responsiveness.
  • MHC and disease susceptibility.

 

 

12.00
Unit IV: 
UNIT IV

Antigen Processing and Presentation.

  • Role of antigen presenting cell.
  • The cytosolic pathways.Endogenous antigens :

·         The endocytic pathway Exogenous antigens :.

·     Presentation of nonpeptide bacterial antigens.

Cytokines: general structure properties and functions

·    Cytokine related diseases.

 

Immune system in Health and Disease.

·                Immune response to infectious disease

o    Viral infections

o    Bacterial infections

o    Protozoa and diseases

Diseases caused by helminthes

12.00
Unit V: 
UNIT V

Vaccine.

  • Active and passive immunization
  • Types of vaccines

 

AIDS and other immunodeficiencies.

Autoimmunity: Proposed mechanisms for induction of autoimmunity

  • Systemic autoimmune diseases

 

  • Organ specific autoimmune disease

 

Essential Readings: 

 

  • Alberts B, Bray D, LewioJ, RaffM, Roberts K and Watson J.D. Molecular Biology of the Cell. Garland Publishing, Inc. New York, 1994.
  • Glick B. R. and Pasternak 1.1. Molecular Biotechnology Principles and Applications of Recombinant DNA. ASM Press, Washington, 1998.
  • Bolrover S.R. Hyams J.S., Jones S. Shephard E.A. and White H.A. From genes to cells. Wiley-liss, New York, 1997.
  • Winnacker E.L. From genes to clones Introduction to gene technology Panima Education BookAgency, New Delhi. æ VCH Publishers, New York, 1987.
  • Giese A. C. Cell Physiology, WB. Saunders Co., Philadelphia, 1979
  • Freifelder D. Molecular Biology, Naroja Publishing House, New Delhi, 1997.
  • Frieerg E.C., Walker G.C. and Siede, W DNA Repair and Mutagenesis. ASM Press Wadbington DC, 1995.
  • Karp  Cell and Molecular Biology. Concepts and experiments. John Wiley and sons. Inc. New York, 1999

 

Academic Year: 
2015-2016
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