Cell and Molecular Biology II

Paper Code: 
ZOL-423(B)
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
12.00

Molecular Immunology.

Humoral and Cell mediated immunity.

  • Innate (nonspecific) immunity.
  • Adaptive (specific) Immunity.

 

Cells and organs of immune system.Hematopoiesis

  • B-lymphocytes, T-lymphocytes, Null cells.
  • Mononuclear cells
  • Granulocytic cells     
  • Mast Cells.
  • Dendritic cells.

Organs of immune system.

Lymphatic system.

  • Primary lymphoid organs
  • Secondary    lymphoid    organs   

Antigens.

  • Immunogenicity versus antigenicity.
  • Factors  influence immnogenicity
  • Contribution   of  Biological   system. (Genotype   of  the recipient    animal,    immunogen    dosage    and    route    of
    administration, adjuvants).
  • Epitopes
  • Haptans.
12.00

Immunoglobulins : Structure and function.

  • Molecular structure of Ig, Light chain and Heavy chain
  • Immunoglobulin domains.

 

  • Immunoglobulin classes
  • Immunoglobulin-mediated     effector    functions                                     
  • Antigenic determinants on immunoglobulin (isotype, allotype and idiotype).

 

Generation of antibody diversity.

  • Multiple germ line V, D and J gene segments.
  • Combinatorial V-J and V-D-J joining.
  • Junctional flexibility.
  • P-addition and N-addition.
  • Association of heavy and light chain.

Class switching among constant region genes.

  • Expression of Ig genes.
  • Regulation of Ig gene transcription.
  • Antibody genes and antibody engineering.
12.00

Antigen-Antibody Interaction.( in vivo and in vitro)

Major Histocompatibility complex.

·     General organization and inheritance of MHC.

·     Genomic maps of MHC genes.

·     MHC molecules and genes.

 

·      Cellular distribution of MHC molecules.

·            Regulation of MHC expression.

·      MHC and immune responsiveness.

·      MHC and disease susceptibility.

 

12.00

Antigen Processing and Presentation.

  • Role of antigen presenting cell.
  • The cytosolic pathways.Endogenous antigens :
  • The endocytic pathway Exogenous antigens :.
  • Presentation of nonpeptide bacterial antigens.

Cytokines: general structure properties and functions

  • Cytokine related diseases.

 

Immune system in Health and Disease.

  • Immune response to infectious disease
    • Viral infections
    • Bacterial infections
    • Protozoa and diseases
    • Diseases caused by helminthes
12.00

Vaccine.

  • Active and passive immunization
  • Types of vaccines

 

AIDS and other immunodeficiencies.

Autoimmunity: Proposed mechanisms for induction of autoimmunity

  • Systemic autoimmune diseases

 

  • Organ specific autoimmune disease
Essential Readings: 
  • Alberts B, Bray D, LewioJ, RaffM, Roberts K and Watson J.D. Molecular Biology of the Cell. Garland Publishing, Inc. New York, 1994.
  • Glick B. R. and Pasternak 1.1. Molecular Biotechnology Principles and Applications of Recombinant DNA. ASM Press, Washington, 1998.
  • Bolrover S.R. Hyams J.S., Jones S. Shephard E.A. and White H.A. From genes to cells. Wiley-liss, New York, 1997.
  • Winnacker E.L. From genes to clones Introduction to gene technology Panima Education BookAgency, New Delhi. æ VCH Publishers, New York, 1987.
  • Giese A. C. Cell Physiology, WB. Saunders Co., Philadelphia, 1979
  • Freifelder D. Molecular Biology, Naroja Publishing House, New Delhi, 1997.
  • Frieerg E.C., Walker G.C. and Siede, W DNA Repair and Mutagenesis. ASM Press Wadbington DC, 1995.
  • Karp  Cell and Molecular Biology. Concepts and experiments. John Wiley and sons. Inc. New York, 1999
  • Malacinski G.M. and Frifeilder D. Essentials of Molecular Biology Jones and Bartlett Publishers Boston, 1999
  • Cooper, G .M. The cell A molecular approach. ASM Press, Washington DC, 2000.
  • Shelve P. and Blanch DEW, Cell and Molecular Biology. John Wile) and Sons Inc., New York, 1994.
  • Darnell, J.L. Lodish, H and Baltimore, D. Molecular Cell Biology, Scien tific American Books Inc. New York.
  • Watson, J.D., Hopkins, N.H. Roberts, J.W Steitz, J.A. and Weiner A.M. Molecular Biology of the Gene. The Benjamin/Cumings Pub. Co. Inc. California
  • Gardner, EJ. Simons, MJ. and Snustad, D.P. Principles of Genetics. John Wiley .& Sons Inc. New York
  • Voet, D. and Voet, J.G. Biochemistry, John Wiley & Sons Inc New York.
  • Zubay, G.L. Parson, WW and Vance, D.E. Principles of Biochemistry. Wm.C. Brown Publishers, Oxford, England.
  • Goldsby, RA.. Kindt, T.J. and Osborne, B.A. Kuby Immunology, WH Free- I man & Co. New York.
  • Abbas. AK Lichtman, AR. and Pahes, J.S. Molecular Immunology, WB Saunders & Co, London
  • Cruse. J.M. and Lewis, RE. Atlas of Immunology, CRC Press,New York.
  • Talwar, G.P. and Gupta. S.K A HandbookofPractical and Clinical Immu nology. Vall & II. Vikas Publishing House Pvt. Ltd. NewDelhi.
  • Dabre, D.D. Introduction to Practical Molecular Biology, John Wiley & Sons lnc; New York.
  • Micklos D.A. and Freyer G. A. DNA science A first course in recombinantDNA Techanology. Carolina Biological supply compo and Cold Spring Harbour Lahovatoy Press, Burhington , North corolina, 1990.
  • Lucent Micklos D.A. and Freyer G. A. DNA science A first course in recombinantDNA Techanology. Carolina Biological supply compo and Cold Spring Harbour Lahovatoy Press, Burhington , North corolina, 1990B. Genes VII. Oxford University Press, Oxford, 2000.
  • Old R. W. and Primrose S. B. Principles of Gene Manipulation An introduction to genetic engineering. Blackwell Scientific Pub li- cation. London, 1989.
  • Twyman R. M. and Wisden W. Advanced Molecular Biology A Concise Reference Viva Books Pvt. Ltd. New Delhi: 1999
  • Gasque, C. E. A. Manual of laboratory experiences in Cell Biology Uni versal Book Stall New Delhi.
  • Meyers R.A. Molecular Biology and Biotechnology A comprehen- sive desk Reference. VCH Publishers, Inc. New York, 1995
  • Lodish H, BerkA, Zipurjky S. L., Matsudaira P, Baltimore D. and Darnell 3. Molecular Cell Biology W.H. freeman and company New York, 2000.
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