CELL AND MOLECULAR BIOLOGY I (Practical)

Paper Code: 
25ZOL324B
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
This course will enable the students to -
1. Examine working and principle of microscope and coulometer.
2. Hand on experience of various molecular biology techniques.
3. Experimentation on various chromosomal techniques.
4. Discover morphology of different types of cells and tissues.
Course Outcomes: 

Course

Learning outcomes (at course level)

Learning and teaching strategies

Assessmen t Strategies

Course Code

Course Title

 

 

 

 

25ZOL

324B

 

 

 

 

Cell and Molecular Biology (Practical)

CO121. Deduce working and

principle of microscope and

oculometer.

CO122. Practical experience of  various

molecular biology techniques.

CO123.    Develop skills of various                chromosomal techniques and

examine morphology of different types of cells and tissues

CO124. Perceive knowledge about Chromosomes in chironomus larva and mammalian chromosomes from bone marrow.

CO125:Demonstrate skills and record the concepts  in the form  of practical file  and

reflect  on  personal learning experiences and experimental outcomes during viva voce.

CO126. Contribute effectively in course-

specific interaction

Approach in

teaching:

Practical,

Demonstration

Learning

activities for the

students: Practical knowledge, Effective Questions

Class test

Semester

end

examinatio

ns

Viva voce

 

● Use of microscopes
● Use of oculometer: standardization and measurements of cell height,nuclear diameter and tubular diameters
● Determination of the absorption maxima of a solution.
● Determination of relationship between absorption and various concentration of a solution using colorimeter and spectrophotometer.
● Preparation of standard curve for protein,lipid,carbohydrate and enzymes.
● Separation Techniques
● Separation of proteins and DNA by Agarose gel electrophoresis
● Separation of proteins on SDS PAGE and PAGE
● Separation of amino Acids by Course chromatography
● Separation of amino Acids by TLC 
 
Academic Year: 
2025-2026
  • Printer-friendly version
  • PDF version