CELL AND MOLECULAR BIOLOGY I (Practical)

Paper Code: 
24ZOL324B
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
Course objectives:
This course will enable the students to -
1. Examine working and principle of microscope and coulometer.
2. Hand on experience of various molecular biology techniques.
3. Experimentation on various chromosomal techniques.
4. Discover morphology of different types of cells and tissues.
 
Course Outcomes: 

Course

Learning outcomes (at course level)

Learning and teaching strategies

Assessmen t Strategies

Course Code

Course Title

 

24ZOL

324B

Cell and Molecular Biology (Practical)

 

CO121. Deduce working and

principle of microscope and

oculometer.

CO122. Practical experience of  various

molecular biology techniques.

CO123.    Infer    various    chromosomal

techniques and examine morphology of different types of cells and tissues

CO124. Perceive knowledge about Chromosomes in chironomus larva and mammalian chromosomes from  bone

marrow.

CO125.  Reflect  on  personal  learning

experiences and experimental outcomes

during viva voce assessments.

CO126. Contribute effectively in course-

specific interaction

Approach in

teaching:

Practical,

Demonstration

Learning

activities for the

students: Practical knowledge, Effective Questions

Class test

Semester

end

examinatio

ns

Viva voce

 

CONTENTS
● Use of microscopes
● Use of oculometer: standardization and measurements of cell height,nuclear diameter and tubular diameters
● Determination of the absorption maxima of a solution.
● Determination of relationship between absorption and various concentration of a solution using colorimeter and spectrophotometer.
● Preparation of standard curve for protein,lipid,carbohydrate and enzymes.
● Separation Techniques
● Separation of proteins and DNA by Agaose gel electrophoresis
● Separation of proteins on SDS PAGE and PAGE
● Separation of amino Acids by Course chromatography
● Separation of amino Acids by TLC
 
● Chromosomal Techniques
● Preparation of salivary gland chromosome from chironomus larva and stained with acetocarmine /aceto orcein and feulgen
● Preparation of mammalian chromosomes from bone marrow
● Permanent slides-Types of cells(Squamous,cuboidal,columnar epithelial cells,Nerve Cells, Muscle Cells,Connective tissues of various types),adipose tissues,mitotic and meiotic chromosomes and their different phases.
 
Essential Readings: 
• Shelve P. and Blanch DEW, Cell and Molecular Biology. John Wile) and Sons Inc., New York, 1994.
• Darnell, J.L. Lodish, H and Baltimore, D. Molecular Cell Biology, Scien tific American Books Inc. New York.
• Watson, J.D., Hopkins, N.H. Roberts, J.W Steitz, J.A. and Weiner A.M. Molecular Biology of the Gene. The Benjamin/Cumings Pub. Co. Inc. California
• Gardner, EJ. Simons, MJ. and Snustad, D.P. Principles of Genetics. John Wiley .& Sons Inc. New York
• Voet, D. and Voet, J.G. Biochemistry, John Wiley & Sons Inc New York.
• Zubay, G.L. Parson, WW and Vance, D.E. Principles of Biochemistry. Wm.C. Brown Publishers, Oxford, England.
• Goldsby, RA.. Kindt, T.J. and Osborne, B.A. Kuby Immunology, WH Free- I man & Co. New York.
 
References: 
SUGGESTED READINGS:
• Old R. W. and Primrose S. B. Principles of Gene Manipulation An introduction to genetic engineering. Blackwell Scientific Pub li- cation. London, 1989.
• Twyman R. M. and Wisden W. Advanced Molecular Biology A Concise Reference Viva Books Pvt. Ltd. New Delhi: 1999
• Gasque, C. E. A. Manual of laboratory experiences in Cell Biology Universal Book Stall New Delhi.
• Meyers R.A. Molecular Biology and Biotechnology A comprehen- sive desk Reference. VCH Publishers, Inc. New York, 1995
E- RESOURCES
Chromeextension://efaidnbmnnnibpcajpcglclefindmkaj/https://faculty.ksu.edu.sa/sit es/default/files/bch361_handnote_1.pdf
JOURNALS:
 
Academic Year: 
2024-2025
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