Coming soon
Cell and molecular Biology I (Practical)
Course Outcomes (COs):
|
Course |
Learning outcomes (at course level) |
Learning and teaching strategies |
Assessment Strategies |
|
|
Paper Code |
Paper Title |
|||
|
ZOL 324B |
Cell and molecular Biology I (Practical) |
Students will – CO101: Deduce working and principle of microscope and oculometer. CO102: Practical experience of various molecular biology techniques. CO103: Infer various chromosomal techniques. CO104: Conclude morphology of different types of cells and tissues CO105: Perceive knowledge about Chromosomes in chironomus larva and mammalian chromosomes from bone marrow. |
Approach in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching
Learning activities for the students: Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Field practical
|
Class test Semester end examinations, Quiz Assignments, Presentations Surprise test Open book test |
- Use of microscopes
- Use of oculometer: standardization and measurements of cell height,nuclear diameter and tubular diameters
- Use of ocular Grid-standardization and counting of cells or nuclei in cross sectional epithelium.
- Determination of the absorption maxima of a solution.
- Determination of relationship between absorption and various concentration of a solution using colorimeter and spectrophotometer.
- Preparation of standard curve for protein ,lipid ,carbohydrate and enzymes.
- Separation Techniques
- Separation of proteins and DNA by Agaose gel electrophoresis
- Separation of proteins on SDS PAGE and PAGE
- Separation of amino Acids by paper chromatography
- Separation of amino Acids by TLC
- Chromosomal Techniques
- Preparation of salivary gland chromosome from chironomus larva and stained with acetocarmine /aceto orcein and feulgen
- Preparation of mammalian chromosomes from bone marrow
- Permanent slides-Types of cells(Squamous,cuboidal,columnar epithelial cells,Nerve Cells, Muscle Cells,Connective tissues of various types),adipose tissues,mitotic and meiotic chromosomes and their different phases, Cancer cells of various types.
