Coming soon
CELL AND MOLECULAR BIOLOGY I
Paper Code:
24ZOL323B
Credits:
4
Contact Hours:
4.00
Max. Marks:
100.00
Objective:
Course objectives:
This course will enable the students to -
1. Formulate fundamental knowledge of the cellular components, functions and regulatory pathways of the cell cycle emphasizing their significance in controlling cancer, aging and apoptosis.
2. Enlighten the students about life processes at the molecular levels. It includes the molecular structure of genes and chromosomes and regulation of post transcriptional and posttranslational gene expression.
Course Outcomes:
|
Course |
Learning outcomes (at course level) |
Learning and teaching strategies |
Assessment Strategies |
|
|
Course Code |
Course Title |
|||
|
24ZOL 323B |
Cell And Molecula r Biology (Theory)
|
CO91. Examine the structure and function of biomembrane and its role in active and passive transport. CO92. Elaborate the role of Cytoskeleton and cell signaling CO93. Acquire the detailed knowledge of different cell Junctions and cellular adhesion molecules and cell cycle and its regulation CO94. Perceive the detailed knowledge of cancer, its mechanism, ageing and apoptosis CO95. Conclude the role of chromosomal organization of genes and noncoding DNA, DNA binding domains and regulation of gene expression. CO96. Contribute effectively in course-specific interaction |
Approach in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching Learning activities for the students: Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks |
Class test Semester end examinations , Quiz Assignments , Presentation Surprise test Open book test |
12.00
Unit I:
UNIT I
Molecular structure of genes and chromosomes
● Molecular definition of gene.
● Chromosomal organization of genes and non-coding DNA.
● Mobile DNA.
● Functional rearrangement of chromosomal DNA
● Mutation: Types, causes and detection, mutant types – lethal, conditional,
biochemical, loss of function, gain of function, germinal vs somatic mutants, insertional mutagenesis.
● Structural and numerical alterations of chromosomes: Deletion, duplication, inversion, translocation, ploidy and their genetic implications.
12.00
Unit II:
Unit II
Regulation of Gene Expression
● Operon Concept.
● Catabolite Repression.
● Positive and Negative regulation.
● Inducers and co-repressors.
● Attenuation mechanism
DNA binding & Protein dimerization domains.
● Homeodomain proteins.
● Zinc finger proteins.
● Leucine-Zipper proteins.
● Helix-loop-helix proteins
12.00
Unit III:
Unit III
Cell cycle
● Eukaryotic cell cycle-Gl, S, G2 and M Phases.
● Cell cycle and checkpoints.
● Molecular basis of cell cycle regulation.
● Cyclins and cyclin-dependent kinases.
● Regulation of CDK-cyclin activity
Cell Death: Apoptosis and necrosis
● Extrinsic pathway
● Intrinsic pathway
● apoptotic and antiapoptotic genes
12.00
Unit IV:
Unit IV
Cancer
● Properties of cancer cell and onset of cancer
● Causes of cancer
● Retrovirus
● Mechanism of conversion of Proto-oncogenes to oncogenes
● Tumor suppressor genes
Aging: The biology of senescence:
● Maximum life span and life expectancy
● causes of aging
12.00
Unit V:
Unit V
Mendelian principles: Dominance, segregation, independent assortment
● Interaction of genes: Codominance, incomplete dominance, dominant and recessive epistasis, complementary genes, supplementary genes, duplicate genes, lethal genes
● pleiotropy, penetrance and expressivity, linkage and crossing over, sex linkage, sex limited and sex influenced characters.
● Pedigree analysis:
● autosomal recessive traits-
● autosomal dominant traits:
● X linked recessive traits
● X linked dominant traits
● Y linked traits
Essential Readings:
● IWASA, J., MARSHALL, W. F., & KARP, G. (2016). Karp's cell and molecular biology: concepts and experiments.
● COOPER, G. M., & HAUSMAN, R. E. (2009). The cell: a molecular approach. Washington, D.C., ASM Press.
● Nelson et al, Lehninger’s Principles of Biochemistry, 4th Edition, McMillan, 2006.
● Alberts et al, Molecular Biology of The Cell, 2nd Edition , Garland 2007.
● Lewin, Genes IX, 9th Edition , Jones & Bartlett, 2007
● .Molecular Biology of the Cell ; Lewis and Watson
● Genes VII : Benjamin Lewin
References:
SUGGESTED READINGS:
● Pastemak, An Introduction to Molecular Human Genetics, 2nd Edition, Fritzgarald, 2005.
● Strachen and Read, Human Molecular Genetics, 3rd Edition, Garland Sci. Publishing, 2004.
● Structural Chemistry and Molecular Biology Alexander Rich and Norman Davidson
● The Chemical Foundations of Molecular :James D. Watson
● Human Cytogenetic Techniques and Clinical Applications HemaPurandare and Arnit Chakravarty
● Progress in Nucleic Acid Research and Molecular Biology Vol. 9 J. N. Davidson
● Essentials of Human Genetics Bhatnagar,Kothari,Mehta
● Principles and Practice of Medical Genetics, by Emery, A.E.H and D.L. Rimoin (Eds_ (1990-2nd edition) Churchill Livingstone, Edinburgh.
● Molecular Basis of Inherited Diseases, (6th Edition-1989) by Scriver, C.R. A.L. Beudit, W.S. Sty ab nd D. Valle (Eds0 McGrawHill, New York.
● Human Genetics by S.D. Gangane (2nd edition-Reprint 2001), B.L Churchill Livingstone Pvt. Ltd., New Delhi.
● Genetics in Medicine by M.W. Thompson et al, 5th Edition, W.B. Sounders Company, London
● Genetic basis of common diseases by R. A. King et al, Oxford University Press.
● Mendelian inheritance in Man by Mc. Kusick V.A. (1998), 12th Edition, John Hopsins University Press, Baltimore.
e- RESOURCES:
● Cell biology -NPTEL- https://nptel.ac.in/courses/102103012
● Molecular Biology-NPTEL-https://nptel.ac.in/courses/102104052
e- JOURNAL:
● Molecular and Cellular Biology
● BMC Cell and Molecular Biology- ISSN: 2661-8850
Academic Year:
