Cell and Molecular Biology I

Paper Code: 
ZOL 323 B
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course objectives:

 

This course will enable the students to -

  1. Formulate fundamental knowledge of the cellular components, functions and regulatory pathways of the cell cycle emphasizing their significance in controlling cancer, aging and apoptosis.
  2. Enlighten the students about life processes at the molecular levels. It includes the molecular structure of genes and chromosomes and regulation of post transcriptional and posttranslational gene expression.

 

Course Outcomes (COs):

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

ZOL 323B

 

Cell And Molecular Biology

Students will –

CO77: Examine the structure and function of biomembrane and its role in active and passive transport.

CO78: Elaborate the role of Cytoskeleton, Cell Junctions and cellular adhesion molecules in intracellular movements and cell to cell and cell to matrix interactions.

CO79: Acquire the detailed knowledge of different pathways related to cell signaling and apoptosis thus enabling them to understand the importance of cell cycle regulation checkpoints.

CO80: Perceive the detailed knowledge about chromosomal organization of genes and noncoding DNA.

CO81: Conclude the role of DNA binding motifs in regulation of gene expression.

Approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching

 

Learning activities for the students:

Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks

 

Class test

Semester end examinations,

Quiz

Assignments,

Presentation

Surprise test

Open book test

 

12.00
Unit I: 
UNIT I
12.00
Unit II: 
UNIT II
Molecular structure of genes and chromosomes
● Molecular definition of gene.
● Chromosomal organization of genes and non-coding DNA.
● Mobile DNA.
● Functional rearrangement of chromosomal DNA
● Mutation: Types, causes and detection, mutant types – lethal, conditional, biochemical, loss of function, gain of function, germinal vs somatic mutants, insertional mutagenesis.
● Structural and numerical alterations of chromosomes: Deletion, duplication, inversion, translocation, ploidy and their genetic implications
 
12.00
Unit III: 
UNIT III

Regulation of Gene Expression

  • Operon Concept.
  • Catabolite Repression.
  • Positive and Negative regulation.
  • Inducers and co-repressors.
  • Attenuation mechanism

DNA binding & Protein dimerization domains.

  • Homeodomain proteins.
  • Zinc finger proteins.
  • Leucine-Zipper proteins.
  • Helix-loop-helix proteins             

 

 

12.00
Unit IV: 
UNIT IV

Cell cycle

  • Eukaryotic cell cycle-Gl, S, G2 and M Phases.
  • Cell cycle and checkpoints.
  • Molecular basis of cell cycle regulation.
  • Cyclins and cyclin-dependent kinases.
  • Regulation of CDK-cyclin activity

Cell Death: Apoptosis and necrosis

  • Extrinsic pathway
  • Intrinsic pathway
  • apoptotic and antiapoptotic genes

 

12.00
Unit V: 
UNIT V
  • Mendelian principles: Dominance, segregation, independent assortment
  • Interaction of genes: Codominance, incomplete dominance, dominant and recessive epistasis, complementary genes, supplementary genes, duplicate genes, lethal genes
  • pleiotropy, penetrance and expressivity, linkage and crossing over, sex linkage, sex limited and sex influenced characters.
  • Pedigree analysis:
  • autosomal recessive traits-
  • autosomal dominant traits:
  • X linked recessive traits
  • X linked dominant traits
  • Y linked traits

 

Essential Readings: 
● IWASA, J., MARSHALL, W. F., & KARP, G. (2016). Karp's cell and molecular biology: concepts and experiments.
● COOPER, G. M., & HAUSMAN, R. E. (2009). The cell: a molecular approach. Washington, D.C., ASM Press.
● Nelson et al, Lehninger’s Principles of Biochemistry, 4th Edition, McMillan, 2006.
● Alberts et al, Molecular Biology of The Cell, 2nd Edition , Garland 2007.
● Lewin, Genes IX, 9th Edition , Jones & Bartlett, 2007
● .Molecular Biology of the Cell ; Lewis and Watson
● Genes VII : Benjamin Lewin
● Lewis, Human Genetics, 7th Edition, WCB & McGraw, 2007
 
References: 
SUGGESTED READINGS:
● Pastemak, An Introduction to Molecular Human Genetics, 2nd Edition, Fritzgarald, 2005.
● Strachen and Read, Human Molecular Genetics, 3rd Edition, Garland Sci. Publishing, 2004.
● Structural Chemistry and Molecular Biology Alexander Rich and Norman Davidson
● The Chemical Foundations of Molecular :James D. Watson
● Human Cytogenetic Techniques and Clinical Applications HemaPurandare and Arnit Chakravarty
● Progress in Nucleic Acid Research and Molecular Biology Vol. 9 J. N. Davidson
● Essentials of Human Genetics Bhatnagar,Kothari,Mehta
● Principles and Practice of Medical Genetics, by Emery, A.E.H and D.L. Rimoin (Eds_ (1990-2nd edition) Churchill Livingstone, Edinburgh.
● Molecular Basis of Inherited Diseases, (6th Edition-1989) by Scriver, C.R. A.L. Beudit, W.S. Sty ab nd D. Valle (Eds0 McGrawHill, New York.
● Human Genetics by S.D. Gangane (2nd edition-Reprint 2001), B.L Churchill Livingstone Pvt. Ltd., New Delhi.
● Genetics in Medicine by M.W. Thompson et al, 5th Edition, W.B. Sounders Company, London
● Genetic basis of common diseases by R. A. King et al, Oxford University Press.
● Mendelian inheritance in Man by Mc. Kusick V.A. (1998), 12th Edition, John Hopsins University Press, Baltimore.
 
e- RESOURCES:
● Cell biology -NPTEL- https://nptel.ac.in/courses/102103012
● Molecular Biology-NPTEL-https://nptel.ac.in/courses/102104052
e- JOURNAL: 
● Molecular and Cellular Biology
● BMC Cell and Molecular Biology- ISSN: 2661-8850
 
Academic Year: 
2022-2023
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