Cell and Molecular Biology I

Paper Code: 
ZOL 323 B
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
Course objectives:
 
This course will enable the students to -
  1. Formulate fundamental knowledge of the cellular components, functions and regulatory pathways of the cell cycle emphasizing their significance in controlling cancer, aging and apoptosis. 
  2. Enlighten the students about life processes at the molecular levels. It includes the molecular structure of genes and chromosomes and regulation of post transcriptional and posttranslational gene expression.
Course Outcomes (COs): 
 
 

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

ZOL 323B

 

Cell And Molecular Biology

The Students will –

CO77: Examine the structure and function of bio membrane and its role in active and passive transport.

CO78: Elaborate the role of Cytoskeleton, Cell Junctions and cellular adhesion molecules in intracellular movements and cell to cell and cell to matrix interactions.

CO79: Acquire the detailed knowledge of different pathways related to cell signalling and apoptosis thus enabling them to understand the importance of cell cycle regulation checkpoints.

CO80: Perceive the detailed knowledge about chromosomal organization of genes and noncoding DNA.

CO81: Conclude the role of DNA binding motifs in regulation of gene expression.

Approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching

 

Learning activities for the students:

Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks

 

Class test

Semester end examinations,

Quiz

Assignments,

Presentation

Surprise test

Open book test

 

12.00
Unit I: 
UNIT I :
Biomembranes
  • Phospholipids : main lipid constituent.
  • Cytosolic and exoplasmic face of biomembranes.
  • Intrinsic and extrinsic proteins.
  •  Glycolipids.
  • Mobility of lipids and integral proteins
  • Fluidity of bio membrane. Transport across cell membrane.
  • Diffusion of small molecules.
  • Osmosis and water channels.
  • Uniporter-catalyzed transport.
  • ActiveTransport.
  • Cotransport by symporters and antiporters.
12.00
Unit II: 
UNIT II :
Cytoskeleton: Structure , Function and Dynamics of
 
  • Microfilaments: Actin andMyosin
  • Microtubule in mitosis.
  • Kinesin andDynein.
  • Intermediate Filaments
 
Cell-Cell Signaling.
  • Endocrine, paracrine, autocrine signaling.
  • Receptor Proteins- Intracellular Receptors.
  • Cell surface receptors-G-protein coupled receptors, ion channel receptors, tyrosine kinase linked receptors and receptors with
  • intrinsic enzymatic activity.
  • Second messenger system-cAMP,IP3-DAG and MAP kinase pathways.
  • Signal-Mediated transport through Nuclear Pore.
  • Nuclear Pore Complex.
  • Nuclear export signals.
  • Nuclear localization signals
12.00
Unit III: 
UNIT III
Cell Junctions : Occluding, Adherence and communicating junctions Cell- cell adhesion molecules
  • Selectin
  • Cadherin
  • Integrin
  • Ig Superfamily
Cell matrix adhesion
  • Integrins
  • Collagen
  • Non-collagen components of extracellular matrix
Cell cycle.
  • Eukaryotic cell cycle- Gl, S, G2 and M Phases.
  • Cell cycle and checkpoints.
  • Molecular basis of cell cycle regulation.
  • Cyclins and cyclin-dependent kinases.
  • Regulation of CDK-cyclin activity
 
12.00
Unit IV: 
UNIT IV
Cancer: Properties of cancer cell and onset of cancer
  • Causes of cancer
  • Retrovirus
  • Mechanism of conversion of Proto-oncogenes to oncogenes
  • Tumor suppressor genes
  • Mutation causing loss of cell cycle. Aging: The biology of senescence.
  • Maximum life span and life expectancy.
  • Causes of aging
  • Cell Death: Apoptosis and necrosis.
 
12.00
Unit V: 
UNIT V
Molecular structure of genes and chromosomes.
  • Molecular definition of gene.
  • Chromosomal organization of genes and non-coding DNA.
  • Mobile DNA.
  • Functional rearrangement of chromosomal DNA
  • Morphological and functional elements of eukaryotic chromosomes.
 
Regulation of Gene Expression.
  • Operon Concept.
  • Catabolic Repression.
  • Positive and Negative regulation.
  • Inducers and co-repressors. DNA binding proteins and gene regulation.
  • DNA binding domains.
  • Homeo  domain proteins.
  • Zinc finger proteins.
  • Winged-helix (Forked head) proteins.
  • Leucine-Zipper proteins.
  • Helix-loop-helix proteins
Essential Readings: 
  • Nelson et al, Lehninger’s Principles of Biochemistry, 4th Edition, McMillan, 2006.
  • Alberts et al, Molecular Biology of The Cell, 2nd Edition , Garland 2007.
  • Lewin, Genes IX, 9th Edition , Jones & Bartlett, 2007.
  • Pastemak, An Introduction to Molecular Human Genetics, 2nd Edition, Fritzgarald, 2005.
  • Lewis, Human Genetics, 7th Edition, WCB & McGraw, 2007..
  • Strachen and Read, Human Molecular Genetics, 3rd Edition, Garland Sci. Publishing, 2004.
  • Structural Chemistry and Molecular Biology Alexander Rich and Norman Davidson
  • The Chemical Foundations of Molecular :James D. Watson
  • Biology of the Gene (2nd ed.)
  • Molecular Biology of the Cell ; Lewis and Watson
  • Genes VI : Benjamin Lewin
  • Human Cytogenetic Techniques and Clinical Applications HemaPurandare and Arnit Chakravarty
  • Progress in Nucleic Acid Research and Molecular Biology Vol. 9 J. N. Davidson
  • Essentials of Human Genetics Bhatnagar,Kothari,Mehta
  • Genes VII :Benjamin Lewin
  • Molecular Biology of the Gene (4th ed.)Watson
  • Molecular Biology of the Cell :Alberts. Lewis & Watson
  • An introduction to genetic Engineering Desmond S. T. Nicoll
  • Principles and Practice of Medical Genetics, by Emery, A.E.H and D.L. Rimoin (Eds_ (1990-2nd edition) Churchill Livingstone, Edinburgh.
  • Molecular Basis of Inherited Diseases, (6th Edition-1989) by Scriver, C.R. A.L. Beudit, W.S. Sty ab nd D. Valle (Eds0 McGrawHill, New York.
  • Human Genetics by S.D. Gangane (2nd edition-Reprint 2001), B.L Churchill Livingstone Pvt. Ltd., New Delhi.
  • Genetics in Medicine by M.W. Thompson et al, 5th Edition, W.B. Sounders Company, London
  • Genetic basis of common diseases by R. A. King et al, Oxford University Press.
  • Mendelian inheritance in Man by Mc. Kusick V.A. (1998), 12th Edition, John Hopsins University Press, Baltimore.
 
Academic Year: 
2021-2022
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