Cell and Molecular Biology

Paper Code: 
ZOL 323 B
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
12.00
Unit I: 
UNIT I

Biomembranes.

  • Phospholipids : main lipid constituent.
  • Cytosolic and exoplasmic face of biomembranes.
  • Intrinsic and extrinsic proteins.

 

  •  Glycolipids.
  • Mobility of lipids and integral proteins
  • Fluidity of bio membrane.
  • Cell Junctions (Gap, tight and desmosomes )

Transport across cell membrane.

  •   Diffusion of small molecules.
  • Osmosis and water channels.
  • Uniporter-catalyzed transport.
  • Active Transport.
  • Cotransport by symporters and antiporters.
 

 

12.00
Unit II: 
UNIT II

Cytoskeleton:Structure ,Function and Dynamics of

·         Microfilaments:Actin and Myosin

·         Microtubule and mitosis.

·                  Kinesin and Dynein.

·                  Intermediate Filaments

Cilia and Flagella.

·         Structure and movements

Cell-Cell Signaling.

·                  Endocrine, paracrine, autocrine signaling.

·         Receptor    Proteins-    Intracellular receptors.
 

     Cell surface receptors-G-protein coupled    receptors, ion channel receptors, tyrosine kinase  linked  receptors and receptors with
intrinsic enzymatic activity.

·         Second messenger system-cAMP and IP3 DAG and MAP kinase pathways.

 

Signal-Mediated transport through Nuclear Pore.

·          Nuclear Pore Complex.

·         Nuclear export signals.
 

Nuclear localization signals 

12.00
Unit III: 
UNIT III

Cell-Cell adhesion and communication.

  • Cadherin mediated Ca- dependent homophilic cell-cell adhesion.
  • N-CAMs mediate Ca-independent homophilic cell-cell adhesion.
  •  Connexin.

Cell matrix adhesion.

  • Integrins
  • Collagen
  • Non-collagen   components   of   extracellular   matrix  

Cell cycle.

  • Eukaryotic cell cycle-Gl, S, G2 and M phases.
  • Cell cycle and check points.
  • Molecular basis of cell cycle regulation.
 

 

12.00
Unit IV: 
UNIT IV

Cancer.

·                  Tumor cells and the onset of cancer.

·                  Proto-oncogenesis and tumor suppressor genes.

·                  Mutation causing loss of cell cycle.

·                  Mutations affecting genome stability.

 

Aging: The biology of senescence.

·                  Maximum life span and life expectancy.

·                  Causes of aging

Cell Death.
 

Apoptosis and necrosis.

12.00
Unit V: 
UNIT V

Molecular structure of genes and chromosomes.

·              Molecular definition of gene.

·              Chromosomal organization of genes and non-coding DNA.

·              Mobile DNA.

·              Functional rearrangement of chromosomal DNA

·              Morphological     and     functional      elements      of     eukaryotic chromosomes.

Regulation of Gene Expression.

·              Operon concept.

·              Catabolic repression.

·              Positive and Negative regulation.

·              Inducers and co-repressors.

 

DNA binding proteins and gene regulation.

·            DNA binding domains.

·            Homeo domain proteins.

·            Zinc finger proteins.

·            Winged-helix (Forked head) proteins.

·            Leucine-Zipper proteins.
 

Helix-loop-helix proteins

Essential Readings: 
  • Nelson et al, Lehninger’s Principles of Biochemistry, 4th Edition, McMillan, 2006.
  • Alberts et al, Molecular Biology of The Cell, 2nd Edition , Garland 2007.
  • Lewin, Genes IX, 9th Edition , Jones & Bartlett, 2007.
  • Pastemak, An Introduction to Molecular Human Genetics, 2nd Edition, Fritzgarald, 2005.
  • Lewis, Human Genetics, 7th Edition, WCB & McGraw, 2007..
  • Strachen and Read, Human Molecular Genetics, 3rd Edition, Garland Sci. Publishing, 2004.
  • Structural Chemistry and Molecular Biology Alexander Rich and Norman Davidson
  • The Chemical Foundations of Molecular :James D. Watson
  • Biology of the Gene (2nd ed.)
  • Molecular Biology of the Cell ; Lewis and Watson
  • Genes VI : Benjamin Lewin
  • Human Cytogenetic Techniques and Clinical Applications HemaPurandare and ArnitChakravarty
  • Progress in Nucleic Acid Research and Molecular Biology Vol. 9 J. N. Davidson
  • Essentials of Human Genetics Bhatnagar,Kothari,Mehta
  • Genes VII :Benjamin Lewin
  • Molecular Biology of the Gene (4th ed.)Watson
  • Molecular Biology of the Cell :Alberts. Lewis & Watson
  • An introduction to genetic Engineering Desmond S. T.Nicoll
  • Principles and Practive of Medical Genetics, by Emery, A.E.H and D.L. Rimoin (Eds_ (1990-2nd edition) Churchill Livingstone, Edinburgh.
  • Molecular Basis of Inherited Diseases, (6th Edition-1989) by Scriver, C.R. A.L. Beudit, W.S. Sty abnd D. Valle (Eds0 McGrawHill, New York.
  • Human Genetics by S.D. Gangane (2nd edition-Reprint 2001), B.L Churchill Livingstone Pvt. Ltd., New Delhi.
  • Genetics in Medicine by M.W. Thompson et al, 5th Edition, W.B. Sounders Company, London
  • Genetic basis of common diseases by R. A. King et al, Oxford University Press.
  • Mendelian inheritance in Man by Mc. Kusick V.A. (1998), 12th Edition, John Hopsins University Press, Baltimore.

 

Academic Year: 
2015-2016
  • Printer-friendly version
  • PDF version