Biotechnology & its Applications

Paper Code: 
ZOL 511
Credits: 
3
Contact Hours: 
45.00
Objective: 

Course objectives:

This course will enable the students to –

  1. Develop an understanding of the fundamental molecular tools and their applications
  2. Apply their knowledge with a problem-solving approach to recommend strategies of genetic engineering for possible applications in the Biotechnology and allied industry.
  3. Envisages concepts, mechanisms, biological designs, functions, and evolutionary significance of genetic modification or manipulation in special organisms and also discusses the recent advance in recombinant DNA technology.

Course Outcomes (COs):

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment

Strategies

Paper Code

Paper Title

ZOL 511

Biotechnology and Its Applications

Students will be able

CO 83: Develop an understanding of the fundamental molecular tools and their applications

CO 84: Apply their knowledge with a problem solving approach to recommend strategies of genetic engineering for possible applications in Biotechnology and allied industry.

CO 85: Envisages concepts, mechanisms, biological designs, functions and evolutionary significance of genetic modification or manipulation in special organisms and also discusses the recent advance in recombinant DNA technology.

Approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, PowerPoint presentations, Explanation by videos

Learning activities for the students:

Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Field practical

 

Class test

Semester end examinations,

Quiz,

Assignments,

Presentation

Surprise test

Open book test

 

 


10.00
Unit I: 
UNIT I

Basic concepts in Genetic Engineering
Enzymology of Genetic engineering- Restriction enzymes, DNA ligase
Cloning vehicles-plasmids, cosmids, lambda phage & yeast plasmids

9.00
Unit II: 
UNIT II

Introduction of cloned genes into the host cells
Transformation
Transduction
Transgenic animals, their uses and biotechnology
Brief account of cloning

5.00
Unit III: 
UNIT III

Analysis & expression of cloned gene in host cell, Southern blotting, Northern blotting, Polymerase chain reaction, DNA probes
Genomic library &cDNA library

11.00
Unit IV: 
UNIT IV

Hybridoma technology, Monoclonal antibodies and their applications
Genomic research-its advantages and disadvantages
Elementary idea of Human Genome Project

10.00
Unit V: 
UNIT V

Biotechnology in Medicine (outline idea only):
Antibiotics
Vaccines
Vitamins.
Artificial Blood.
Potential hazards and regulations of genetic engineering
Molecular Markers (RFLP,RAPD,AFLP)

Essential Readings: 

Essential Readings:

  • R.C. Dubey, A TextBook of Biotechnology, S. Chand and Company, New Delhi.
  • H.D.Kumar, Molecular Biology and Biotechnology, Vikas Publishing House Pvt. Ltd. New Delhi.

Suggested Readings:

  • Molecular Biotechnology. Glick B.R. & Pasternak J. J; ASM Press.
  • Principles of gene manipulation; An introduction to genetic engineering; Old, R.W & Primrose, S.B; Blackwell Science.
  • Elementary biotechnology, Gupta, P.K; Rastogi Puplications; Meerut.
  • D.M. Wear, Immunology ELBS (English) Language Book Society Low Priced edition, Longman Singapore Publishers Ltd.
  • Iyan M. Roitt Essential Immunology ELBS Low Priced Edition Black well Scientific Publications.
  • Immunology; Kuby; W.H; Freeman Publications.

 

Academic Year: 
2020-2021
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