Biotechnology and its Applications

Paper Code: 
ZOL-511
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
Objective: 
Course objectives:  
This course will enable the students to – 
1. Analyse the fundamental molecular tools and their applications 
2. Appraise strategies of genetic engineering for possible applications in the Biotechnology and  allied industry. 
3. Envisages concepts, mechanisms, biological designs, functions, and evolutionary significance of genetic modification or manipulation in special organisms and also discusses the recent  advance in recombinant DNA technology. 
 
Course Outcomes (COs):  
 

Course 

Learning outcomes 

(at course level)

Learning and  teaching strategies

Assessment  Strategies 

Paper  

Code

Paper Title

ZOL 511 

Biotechnolo gy and Its  Applications

Students will 

CO 83: Conjecture the fundamental  molecular tools and their applications CO 84: Formulate strategies of genetic  engineering in Biotechnology and allied  industry. 

CO 85: Relate the concepts,  mechanisms, biological designs,  functions and evolutionary significance  of genetic modification or manipulation  in special organisms and also  discusses the recent advance in  recombinant DNA technology.

Approach in teaching: Interactive Lectures,  Discussion, Tutorials,  Reading assignments,  Demonstration,  

PowerPoint  

presentations,  

Explanation by videos Learning activities for  the students: 

Self-learning  

assignments, Effective  questions, Simulation,  Seminar presentation,  Giving tasks, Field  practical

Class test  

Semester end  examinations, Quiz,  

Assignments,  Presentation 

Surprise test 

Open book test
 
10.00
Unit I: 
UNIT I:
● Basic concepts in Genetic Engineering 
● Enzymology of Genetic engineering- Restriction enzymes, DNA ligase 
● Cloning vehicles-plasmids, cosmids, lambda phage & yeast plasmids 
 
9.00
Unit II: 
UNIT II:
● Introduction of cloned genes into the host cells 
● Transformation 
● Transduction 
● Transgenic animals, their uses and biotechnology 
● Brief account of cloning 
 
5.00
Unit III: 
UNIT III:
● Analysis & expression of cloned gene in host cell, Southern blotting, Northern blotting,  Polymerase chain reaction, DNA probes 
● Genomic library &cDNA library 
11.00
Unit IV: 
UNIT IV:
● Hybridoma technology, Monoclonal antibodies and their applications 
● Genomic research-its advantages and disadvantages 
● Elementary idea of Human Genome Project 
 
10.00
Unit V: 
UNIT V:
● Biotechnology in Medicine (outline idea only): 
Antibiotics 
Vaccines 
Vitamins. 
Artificial Blood. 
● Potential hazards and regulations of genetic engineering 
● Molecular Markers (RFLP,RAPD,AFLP)    
 
Essential Readings: 
Elementary biotechnology, Gupta, P.K; Rastogi Publications; Meerut.
● R.C. Dubey, A TextBook of Biotechnology, S. Chand and Company, New Delhi.
Principles of gene manipulation; An introduction to genetic engineering; Old, R.W
and Primrose, S.B; Blackwell Science.
● H.D.Kumar, Molecular Biology and Biotechnology, Vikas Publishing House Pvt. Ltd.
New Delhi.
 
References: 
SUGGESTED READINGS:
● Molecular Biotechnology. Glick B.R. & Pasternak J. J; ASM Press.
 
e-RESOURCES: 
• NPTEL Biotechnology  https://nptel.ac.in/courses/102103045
 
e-JOURNAL: 
Electronic Journal of Biotechnology- ScienceDirect.com
Biotechnology Journal- Wiley Online Library 
 
Academic Year: 
2022-2023
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