Biotechnology and its Applications

Paper Code: 
ZOL 511
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to –

  1. Analyse the fundamental molecular tools and their applications
  2. Appraise strategies of genetic engineering for possible applications in the Biotechnology and allied industry.
  3. Envisages concepts, mechanisms, biological designs, functions, and evolutionary significance of genetic modification or manipulation in special organisms and also discusses the recent advance in recombinant DNA technology.

 

Course Outcomes (COs): 

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment 

Strategies

Paper Code

Paper Title

ZOL 511

Biotechnology and Its Applications

Students will

CO 83: Conjecture the fundamental molecular tools and their applications

CO 84: Formulate strategies of genetic engineering in Biotechnology and allied industry.

CO 85: Relate the concepts, mechanisms, biological designs, functions and evolutionary significance of genetic modification or manipulation in special organisms and also discusses the recent advance in recombinant DNA technology.

Approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, PowerPoint presentations, Explanation by videos

Learning activities for the students:

Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Field practical

Class test 

Semester end examinations,

Quiz, 

Assignments, 

Presentation

Surprise test

Open book test

 

10.00
Unit I: 
UNIT I
  • Basic concepts in Genetic Engineering
  • Enzymology of Genetic engineering- Restriction enzymes, DNA ligase
  • Cloning vehicles-plasmids, cosmids, lambda phage & yeast plasmids
 

 

9.00
Unit II: 
UNIT II
  • Introduction of cloned genes into the host cells
  • Transformation
  • Transduction
  • Transgenic animals, their uses and biotechnology
  • Brief account of cloning
 

 

5.00
Unit III: 
UNIT III
 
  • Analysis & expression of cloned gene in host cell, Southern blotting, Northern blotting, Polymerase chain reaction, DNA probes
  • Genomic library &cDNA library

 

11.00
Unit IV: 
UNIT IV
  • Hybridoma technology, Monoclonal antibodies and their applications
  • Genomic research-its advantages and disadvantages
  • Elementary idea of Human Genome Project

 

10.00
Unit V: 
UNIT V
  • Biotechnology in Medicine (outline idea only):
  • Antibiotics
  • Vaccines
  • Vitamins.
  • Artificial Blood.
  • Potential hazards and regulations of genetic engineering
  • Molecular Markers (RFLP,RAPD,AFLP)
Essential Readings: 
  • R.C. Dubey, ATextBook of Biotechnology, S. Chand and Company, New Delhi.
  • H.D.Kumar, Molecular Biology and Biotechnology, Vikas Publishing House Pvt. Ltd. New Delhi.
  • Molecular Biotechnology. Glick B.R. & Pasternak J. J; ASM Press.
  • Principles of gene manipulation; An introduction to genetic engineering; Old, R.W & Primrose, S.B; Blackwell Science.
  • Elementary biotechnology, Gupta, P.K; RastogiPuplications; Meerut.
  • D.M. Wear, Immunology ELBS (English) Language Book Society Low Priced edition, Longman Singapore Publishers Ltd.
  • Iyan M. Roitt Essential Immunology ELBS Low Priced Edition Black well Scientific Publications.
  • Immunology; Kuby; W.H; Freeman Publications.
Academic Year: 
2021-2022
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