Biotechnology And Immunology

Paper Code: 
ZOL 501
Contact Hours: 
Max. Marks: 
Unit I: 
  • Basic concepts in Genetic Engineering.
  • Enzymology of Genetic engineering- Restriction enzymes, DNA ligase
  • Cloning vehicles-plasmids, cosmids, lambda phage & yeast plasmids
Unit II: 
  • Analysis & expression of cloned gene in host cell, Southern blotting, Northern blotting, Polymerase chain reaction, DNA probes
  • Genomic library & cDNA library

Methods of Mice Transgenesis  and Applications in brief

Unit III: 
  • Hybridoma technology, Monoclonal antibodies and their applications
  • Brief account of cloning: Genomic research-its advantages and disadvantages
  •  Elementary idea of Human Genome Project
Unit IV: 


  • Innate & adaptive immunity
  • Cells, Tissues and Molecules of immune system
  • Antigen & antibodies- Structure, types, interaction in vivo and in vitro
  • MHC – Class I and Class II Structure and Function


Unit V: 
  • Mechanism of Humoral & Cell mediated  immune response
  • Complement and its action

 AIDS- causes, symptoms, treatment, mode of infection, HIV lifecycle

Essential Readings: 


  • Molecular Biotechnology. Glick B.R. & Pasternak J. J; ASM Press.
  • Principles of gene manipulation; An introduction to genetic engineering; Old, R.W & Primrose, S.B; Blackwell Science.
  • Elementary biotechnology, Gupta, P.K; Rastogi Puplications; Meerut.
  • Immunology; Kuby; W.H; Freeman Publications.
  • R.C. Dubey, A Text Book of Biotechnology, S. Chand and Company, New Delhi.
  • H.D.Kumar, Molecular Biology and Biotechnology, Vikas Publishing House Pvt. Ltd. New Delhi.
  • D.M. Wear, Immunology ELBS (English) Language Book Society Low Priced edition, Longman Singapore Publishers Ltd.
  • Iyan M. Roitt essential Immunology EIBS Low Priced Edition Black well Scientic Publi Kindt, T. J., Goldsby, R.A., Osborne, B. A. and Kuby, J (2006).  Immunology, VI  Edition. W.H. Freeman and Company.
  •  David, M., Jonathan, B., David, R. B. and Ivan R. (2006).  Immunology,VII Edition, Mosby, Elsevier Publication.
  •  Abbas, K. Abul and Lechtman H. Andrew (2003.) Cellular and Molecular  Immunology. V Edition. Saunders Publication.








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