Biotechnology and Immunology

Paper Code: 
ZOL 501
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
Objective: 
Course Objectives: 
 
This course will enable the students to 
1. Acquire basic understanding of tools used in molecular genetics, and their potential  applications to life sciences. 
2. Analyze basic concepts and techniques in genetic engineering and Cloning. 
3. Develop an understanding of biotechnology in medicine. 
4. Illustrate the ability of our immune system to defend against invading  pathogens, concepts of immunity, hypersensitivity, autoimmunity and  
vaccination. 
 
 
 

Course

Learning outcomes 

(at course level)

Learning and 

teaching strategies

Assessment 

Strategies

Paper

Code

Paper Title

ZOL 

501

Biotechnology  and Immunology

The students will

CO50: Evaluate the tools  used in molecular genetics,  and their potential  applications to life sciences.

CO51: Examine the basic  concepts in Genetic 

Engineering and Cloning.  CO52: Deduce the use of  Biotechnology in Medicine  CO53: Conceptualize how  the innate and adaptive  immune responses coordinate  to fight invading pathogens.

CO54: Predict how immune  modulatory strategies can be  used to enhance immune  responses or to suppress  unwanted immune responses  such as might be required in  hypersensitivity reactions,  transplantations or

autoimmune diseases.

Approach in teaching:  Interactive Lectures,  Discussion, Tutorials,  Reading assignments,  Demonstration, Team  teaching

Learning activities for  the students:

Self-learning assignments, Effective  questions, Simulation,  Seminar presentation,  Giving tasks, Field  practical

Class test Semester end examinations Quiz Solving problems in tutorials Assignments Presentation Individual and group 

projects
 
   
   
   

 

9.00
Unit I: 
Unit I
 
● Basic concepts in Genetic Engineering 
● Enzymology of Genetic engineering- Restriction enzymes, DNA ligase 
● Cloning vehicles-Plasmids, Cosmids, Lambda phage and Yeast plasmids 
 

 

9.00
Unit II: 
Unit-II
● Analysis & expression of Cloned gene in host cell, Southern blotting, Northern blotting,  Polymerase chain reaction, DNA probes 
● Genomic library and cDNA library 
● Methods of Mice Transgenesis, Applications in brief. 
 
7.00
Unit III: 
Unit III
 
● Hybridoma technology, Monoclonal antibodies and their applications 
● Brief account of Cloning: Genomic research-its advantages and disadvantages 
● Elementary idea of Human Genome Project 
 
10.00
Unit IV: 
IV
 
mmunity 
● Innate & adaptive immunity 
● Cells, Tissues and organs of immune system 
● Mechanism of Humoral and Cell mediated immune response 
● MHC – Class I and Class II Structure and Function 
 
10.00
Unit V: 
Unit V
 
● Antigen & antibodies- Structure, types 
● Cytokines-types, properties and function 
● Complement system and its action (classical and alternative pathway) 
● AIDS- causes, symptoms, treatment, mode of infection, HIV life cycle
Essential Readings: 
ESSENTIAL READINGS:
Elementary biotechnology, Gupta, P.K; Rastogi Publications; Meerut.
● R.C. Dubey, A TextBook of Biotechnology, S. Chand and Company, New Delhi.
   Principles of gene manipulation; An introduction to genetic engineering; Old, R.W
   and Primrose, S.B; Blackwell Science.
● H.D.Kumar, Molecular Biology and Biotechnology, Vikas Publishing House Pvt. Ltd.
New Delhi.
● Roitt’s essential Immunology, P. Delves, et al., 11th ed., Blackwell Publishing, 2006
● Kindt, T. J., Goldsby, R.A., Osborne, B. A. and Kuby, J (2006). Immunology, VI Edition.  W.H. Freeman and Company. 
 
SUGGESTED READINGS:
● Molecular Biotechnology. Glick B.R. & Pasternak J. J; ASM Press.
● David, M., Jonathan, B., David, R. B. and Ivan R. (2006). Immunology,VII Edition, Mosby,  Elsevier Publication. 
● Abbas, K. Abul and Lechtman H. Andrew (2017) Cellular and Molecular Immunology. V  Edition. Elsevier; 9th edition 
 
 
e-RESOURCES:
 
e-Journal
Immunology- Wiley online Library- ISSN 1365-2567
Scandinavian Journal of Immunology-Online ISSN: 1365-3083
 
Academic Year: 
2022-2023
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