Biotechnology And Immunology

Paper Code: 
ZOL 501
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
10.00
Unit I: 
UNIT I
  • Basic concepts in Genetic Engineering

Enzymology of Genetic engineering- Restriction enzymes, DNA ligase

  • Cloning vehicles-plasmids, cosmids, lambda phage & yeast plasmids

 

9.00
Unit II: 
UNIT II
  • Analysis & expression of cloned gene in host cell, Southern blotting, Northern blotting, Polymerase chain reaction, DNA probes
  • Genomic library & cDNA library
  •  Methods of Mice Transgenesis ,Applications in brief

 

5.00
Unit III: 
UNIT III
  • Hybridoma technology, Monoclonal antibodies and their applications
  • Brief account of cloning: Genomic research-its advantages and disadvantages
  •  Elementary idea of Human Genome Project

 

11.00
Unit IV: 
UNIT IV

Immunity

·              Innate & adaptive immunity

·              Cells, Tissues and Molecules of immune system

·              Antigen & antibodies- Structure, types, interaction in vivo and in vitro

·              MHC -Basic concepts

Cytokines-Basic concepts

10.00
Unit V: 
UNIT V

·              Mechanism of Humoral & Cell mediated  immune response

·              Complement and its action

 AIDS

Essential Readings: 
  • Molecular Biotechnology. Glick B.R. & Pasternak J. J; ASM Press.
  • Principles of gene manipulation; An introduction to genetic engineering; Old, R.W & Primrose, S.B; Blackwell Science.
  • Elementary biotechnology, Gupta, P.K; Rastogi Puplications; Meerut.
  • Immunology; Kuby; W.H; Freeman Publications.
  • R.C. Dubey, A Text Book of Biotechnology, S. Chand and Company, New Delhi.
  • H.D.Kumar, Molecular Biology and Biotechnology, Vikas Publishing House Pvt. Ltd. New Delhi.
  • D.M. Wear, Immunology ELBS (English) Language Book Society Low Priced edition, Longman Singapore Publishers Ltd.
  • Iyan M. Roitt essential Immunology EIBS Low Priced Edition Black well Scientic Publications
Academic Year: 
2015-2016
  • Printer-friendly version
  • PDF version