BIOTECHNOLOGY AND IMMUNOLOGY

Paper Code: 
ZOL-501
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
Objective: 

Course Objectives:

This course will enable the students to

1. The course objective is to provide students with a basic understanding of tools used in molecular genetics, and their potential applications to life sciences

2. Understand basic concepts and techniques in genetic engineering and Cloning

3. Learn about uses of biotechnology in medicine

4. Understand ability of our immune system to defend against invading pathogens, concepts of immunity, hypersensitivity, autoimmunity and vaccination.

Course Outcomes (COs):

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment

Strategies

Paper Code

Paper Title

ZOL501

BIOTECHNOLOGY AND IMMUNOLOGY

The students will be able to –

CO50: Describe the tools used in molecular genetics, and their potential applications to life sciences.

CO51: Understand the basic concepts in Genetic Engineering and COning

CO52: Describe use of Biotechnology in Medicine

CO53: Conceptualize how the innate and adaptive immune responses coordinate to fight invading pathogens.

CO54: Determine how immunomodulatory strategies can be used to enhance immune responses or to suppress unwanted immune responses such as might be required in hypersensitivity reactions, transplantations or autoimmune diseases.

Approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching

Learning activities for the students:

Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Field practical

Class test

Semester end examinations

Quiz

Solving problems in tutorials

Assignments Presentation

Individual and group projects

 

 

9.00
Unit I: 
UNIT I
Basic concepts in Genetic Engineering
 
Enzymology of Genetic engineering- Restriction enzymes, DNA ligase
 
Cloning vehicles-Plasmids, Cosmids, Lambda phage and Yeast plasmids

 

9.00
Unit II: 
UNIT II
Analysis & expression of cloned gene in host cell, Southern blotting, Northern blotting, Polymerase chain reaction, DNA probes
 
Genomic library and cDNA library
 
 Methods of Mice Transgenesis ,Applications in brief

 

7.00
Unit III: 
UNIT III
Hybridoma technology, Monoclonal antibodies and their applications
 
Brief account of cloning: Genomic research-its advantages and disadvantages
 
Elementary idea of Human Genome Project 

 

10.00
Unit IV: 
UNIT IV
Immunity
 
Innate & adaptive immunity
 
Cells, Tissues and organs of immune system
 
Mechanism of Humoral and  Cell mediated  immune response
 
MHC – Class I and Class II Structure and Function
10.00
Unit V: 
UNIT V
Antigen & antibodies- Structure, types 
 
Cytokines-types, properties and function
 
Complement system and its action (classical and alternate pathway)
 
AIDS- causes, symptoms, treatment, mode of infection, HIV lifecycle

 

Essential Readings: 
Principles of gene manipulation; An introduction to genetic engineering; Old, R.W & Primrose, S.B; Blackwell Science. 
 
Elementary biotechnology, Gupta, P.K; Rastogi Publications; Meerut.
 
Immunology; Kuby; W.H; Freeman Publications.
 
R.C. Dubey, A TextBook of Biotechnology, S. Chand and Company, New Delhi

 

References: 
H.D.Kumar, Molecular Biology and Biotechnology, Vikas Publishing House Pvt. Ltd. New Delhi. 
 
Molecular Biotechnology. Glick B.R. & Pasternak J. J; ASM Press.
 
D.M. Wear, Immunology ELBS (English) Language Book Society Low Priced edition, Longman Singapore Publishers Ltd. 
 
Iyan M. Roitt Essential Immunology ELBS Low Priced Edition Black well Scientific Publications, T. J., Goldsby, R.A., Osborne, B. A. and Kuby, J (2006).  Immunology, VI  Edition. W.H. Freeman and Company. 
 
 David, M., Jonathan, B., David, R. B. and Ivan R. (2006).  Immunology,VII Edition, Mosby, Elsevier Publication. 
 
 Abbas, K. Abul and Lechtman H. Andrew (2003.) Cellular and Molecular  Immunology. V Edition. Saunders Publication.

 

Academic Year: 
2020-2021
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