BIOTECHNOLOGY AND IMMUNOLOGY

Paper Code: 
ZOL-501
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
10.00

·             Basic concepts in Genetic Engineering

Enzymology of Genetic engineering- Restriction enzymes, DNA ligase

·             Cloning vehicles-plasmids, cosmids, lambda phage & yeast plasmids

9.00
  • Analysis & expression of cloned gene in host cell, Southern blotting, Northern blotting, Polymerase chain reaction, DNA probes
  • Genomic library & cDNA library
  •  Methods of Mice Transgenesis ,Applications in brief
5.00

·              Hybridoma technology, Monoclonal antibodies and their applications

·              Brief account of cloning: Genomic research-its advantages and disadvantages

 Elementary idea of Human Genome Project

11.00

Immunity

  • Innate & adaptive immunity
  • Cells, Tissues and Molecules of immune system
  • Antigen & antibodies- Structure, types, interaction in vivo and in vitro
  • MHC -Basic concepts

Cytokines-Basic concepts

10.00
  • Mechanism of Humoral & Cell mediated  immune response
  • Complement and its action
  •  AIDS
Essential Readings: 
  • Molecular Biotechnology. Glick B.R. & Pasternak J. J; ASM Press.
  • Principles of gene manipulation; An introduction to genetic engineering; Old, R.W & Primrose, S.B; Blackwell Science.
  • Elementary biotechnology, Gupta, P.K; Rastogi Puplications; Meerut.
  • Immunology; Kuby; W.H; Freeman Publications.
  • R.C. Dubey, A Text Book of Biotechnology, S. Chand and Company, New Delhi.
  • H.D.Kumar, Molecular Biology and Biotechnology, Vikas Publishing House Pvt. Ltd. New Delhi.
  • D.M. Wear, Immunology ELBS (English) Language Book Society Low Priced edition, Longman Singapore Publishers Ltd.
  • Iyan M. Roitt essential Immunology EIBS Low Priced Edition Black well Scientic Publications.
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