Coming soon
BIOTECHNOLOGY AND IMMUNOLOGY
OBJECTIVES- The course objectives to provide students with a basic understanding of:
- The tools used in molecular genetics, and their potential applications to life sciences.
- Basic concepts in Genetic Engineering and cloning
- Techniques of genetic engineering, Hybridoma technology
- Genomic research and Elementary idea of Human Genome Project
- Use of Biotechnology in Medicine
- The ability of our immune system to defend against invading pathogens in a logical fashion.
- This includes our innate ability to defend against microorganisms (innate immunity); should this first line of defense fail, how we can fight infections (acquired immunity); if we react excessively, what price we pay (hypersensitivity); if we misdirect our defense, what the consequences are (autoimmunity) and very importantly, can we prevent pathogens from infecting us (vaccination).
Learning outcomes-
By the end of the course, students should be able to:
- Describe the tools used in molecular genetics, and their potential applications to life sciences.
- Describe the basic concepts in Genetic Engineering and cloning
- Describe the techniques of genetic engineering.
- Describe Genomic research and outcomes of Human Genome Project
- Describe use of Biotechnology in Medicine
- Conceptualize how the innate and adaptive immune responses coordinate to fight invading pathogens.
- Determine what immunomodulatory strategies can be used to enhance immune responses or to suppress unwanted immune responses such as might be required in hypersensitivity reactions, transplantations or autoimmune diseases.
- Critically review the sample literature to determine the strengths and weaknesses of the data published in immunology and its novelty.
- Explore strategies to improve existing vaccines and how to approach these.
- Basic concepts in Genetic Engineering
Enzymology of Genetic engineering- Restriction enzymes, DNA ligase
Cloning vehicles-Plasmids, Cosmids, Lambda phage &Yeast plasmids
· Analysis & expression of cloned gene in host cell, Southern blotting, Northern blotting, Polymerase chain reaction, DNA probes
· Genomic library &cDNA library
Methods of Mice Transgenesis ,Applications in brief
- Hybridoma technology, Monoclonal antibodies and their applications
- Brief account of cloning: Genomic research-its advantages and disadvantages
Elementary idea of Human Genome Project
Immunity
· Innate & adaptive immunity
· Cells, Tissues and organs of immune system
· Mechanism of Humoral& Cell mediated immune response
MHC – Class I and Class II Structure and Function
- Antigen & antibodies- Structure, types
- Cytokines-types, properties and function
- Complement system and its action (classical and alternate pathway)
AIDS- causes, symptoms, treatment, mode of infection, HIV lifecycle
- Principles of gene manipulation; An introduction to genetic engineering; Old, R.W & Primrose, S.B; Blackwell Science.
- Elementary biotechnology, Gupta, P.K; RastogiPuplications; Meerut.
- Immunology; Kuby; W.H; Freeman Publications.
- R.C. Dubey, A Text Book of Biotechnology, S. Chand and Company, New Delhi.
- H.D.Kumar, Molecular Biology and Biotechnology, Vikas Publishing House Pvt. Ltd. New Delhi.
- Molecular Biotechnology. Glick B.R. & Pasternak J. J; ASM Press.
- D.M. Wear, Immunology ELBS (English) Language Book Society Low Priced edition, Longman Singapore Publishers Ltd.
- Iyan M. Roitt essential Immunology EIBS Low Priced Edition Black well ScienticPubliKindt, T. J., Goldsby, R.A., Osborne, B. A. and Kuby, J (2006). Immunology, VI Edition. W.H. Freeman and Company.
- David, M., Jonathan, B., David, R. B. and Ivan R. (2006). Immunology,VII Edition, Mosby, Elsevier Publication.
- Abbas, K. Abul and Lechtman H. Andrew (2003.) Cellular and Molecular Immunology. V Edition. Saunders Publication.
