Biological Tools and Techniques

Paper Code: 
ZOL-313
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
9.00
Microscopy 
Magnification and Resolving Power
Light (bright-field, dark-field and phase contrast) 
Electron (SEM and TEM)
9.00
Unit II: 
UNIT II

Principles and use of analytical instruments: centrifugation, spectrophotometer, pH meter.

9.00
Unit III: 
UNIT III
Chromatography (Paper & TLC),
Eelectrophoresis (Gel), 
PCR,
 ELISA
9.00
Unit IV: 
UNIT IV
The Internet and the Biologists
The Gene bank sequence database
Structure data base
Sequence analysis using GCG
Information retrieval from Biological database
NCBI data  model
Sequence Alignment and database searching Practical aspects of multiple sequence alignment
Phylogenetic analysis
9.00
Unit V: 
UNIT V
Computer Basics
MS-Office: MS. Word, Power Point and Excel
Hardware and Software
Multimedia
Network Concepts(LAN,WAN)
Internet
Web Servers, Web Access
Essential Readings: 
Cell and Molecular Biology, (3rd edition), P Sheeler and D E Bianchi, John Wiley & Sons, Inc, 1987
Cell and Molecular Biology: Concepts and Experiments, (4th edition), G  Karp, John Wiley & sons, Inc., 2005.
Introduction to Instrumental analysis; Robert Braun:, Mc –Graw hill,
Essentials of Biophysics, P Narayanan, New Age Int. Pub. New Delhi. 2000
Fundamental Laboratory Approaches for Biochemistry and Biotechnology, A J Ninfa., D P Ballou,  Fitzgerald science press, Inc., 1998
Principles and Practice of Bioanalysis, R F Venn, Taylor and Francis, 2003
Principles and Techniques  of Biochemistry and  Molecular Biology ,(6th edition), K Wilson and J Walker (editor), Cambridge University Press, 2007
Bioinstrumentation, J G Webster,  John Wiley & Sons Inc. 2004     
Methods in Modern Biophysics, B Notting, Springer Verlag Berlin Heidelberg New York, 2003
Protein Purification Principles and Practice, (3rd edition), R K Scopes, Spring International, 2004
Spectroscopy for the Biological Sciences, G G Hames, John Wiley & Sons Inc. 2005   
 
Academic Year: