Coming soon
Biological Tools and Techniques
Course objectives:
This course will enable the students to -
- A descriptive study of various techniques used in biological sciences.
- Working and applications of various types of Microscopic techniques and their advantages and disadvantages.
- Explanation of working principles, instrumentation and applications of various analytical instruments like centrifugation, spectrophotometer, pH meter, Chromatography, Electrophoresis, PCR and ELISA.
- Concepts of bioinformatics and its applications, learn to retrieve information from biological databases along with the submission of sequences into the databases and use of general computational tools in the field of Biology
Course Outcomes (COs):
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Course |
Learning outcomes (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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Paper Code |
Paper Title |
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ZOL-313 |
Biological Tools and Techniques |
The students will be able to CO 53: Describe various techniques used in biological sciences. CO 54: Understand the functioning and applications of various types of Microscopic techniques and their advantages and disadvantages. CO 55: Describe working principles, instrumentation and applications of various analytical instruments like centrifugation, spectrophotometer, pH meter, Chromatography, Electrophoresis, PCR and ELISA. CO 56: Gain knowledge about the concepts of bioinformatics and its applications, learn to retrieve information from biological databases along with the submission of sequences into the databases and use of general computational tools in the field of Biology.
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The approach in teaching: Interactive Lectures, Discussion, Tutorials, Guest lectures, PowerPoint presentations, Demonstration of working of various instruments
Learning activities for the students: Self learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks,
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Microscopy
Magnification and Resolving Power
Principle and Application of Microscopy
- Light (bright-field, dark-field and phase contrast)
- Electron (SEM and TEM)
- Fluorescence Microscope
Spectrophotometer (UV- Visible)
Principles and use of analytical instruments:
Centrifugation (Density and Differential)
Chromatography (Paper and TLC)
Electrophoresis (Agarose and PAGE)
PCR
ELISA
The Internet and the Biologists
The Gene bank sequence database
Structure data base
Sequence analysis using GCG
Information retrieval from Biological database
NCBI data model
Sequence Alignment and database searching Practical aspects of multiple sequence alignment
Phylogenetic analysis
Computer Basics
MS-Office: MS. Word, Power Point and Excel
Hardware and Software
Multimedia
Network Concepts(LAN,WAN)
Internet
Web Servers, Web Access
- Wilson and Walker. Practical Biochemistry. Cambridge, 2000.
- Cooper. The Cell-A Molecular Approach. ASM, 1997
- Sharma, Munjal and Shankar. A text book of Bioinformatics. Rastogi Publications, Meerut, UP.
- Essential Bioinformatics, Jin Xiong, John Wiley and Sons. 2006.
Suggested readings:
- Introduction to instrumental analysis-Robert Braun-McGraw Hill.
- A biologist Guide to principles and Techniques of Practical Biochemistry-K, Wilson and K.H. GouldingElBSEdn.
- Essentials of Biophysics, P Narayanan, New Age Int. Pub. New Delhi. 2000.
- Clark &Swizer. Experimental Biochemistry. Freeman, 2000.
- Principles and Practice of Bioanalysis, R F Venn, Taylor and Francis, 2003.
- Locquin and Langeron. Handbook of Microscopy. Butterwaths, 1983
- Boyer. Modern Experimental Biochemistry. Benjamin, 1993
- Freifelder. Physical Biochemistry. Freeman, 1982.
- John R.W. Masters. Animal Cell culture- A practical approach. IRL Press.
- Robert Braun. Introduction to instrumental analysis. McGraw Hill
- David W. Mount's "Bioinformatics" [Cold Spring Harbor Press; ISBN 0879697121].
- James Tisdall. Beginning Perl for Bioinformatics An Introduction to Perl for Biologists. Publisher: O'Reilly Media. October 2001.
