Biological Tools and Techniques

Paper Code: 
ZOL 313
Contact Hours: 
Max. Marks: 


 Magnification and Resolving Power

  • Light (bright-field, dark-field and phase contrast)
  •  Electron (SEM and TEM)
  • Fluorescence Microscope

Principles and use of analytical instruments: centrifugation, spectrophotometer, pH meter.


Principles of Chromatography (Paper,TLC and Liquid-liquid ),

Electrophoresis (Agarose and PAGE)




The Internet and the Biologists

The Gene bank sequence database

Structure data base

Sequence analysis using GCG

Information retrieval from Biological database

NCBI data  model

Sequence Alignment and database searching Practical aspects of multiple sequence alignment

Phylogenetic analysis


Computer Basics

MS-Office: MS. Word, Power Point and Excel

Hardware and Software


Network Concepts(LAN,WAN)


Web Servers, Web Access

Essential Readings: 

Essentials of Biophysics, P Narayanan, New Age Int. Pub. New Delhi. 2000

  • Introduction to instrumental analysis-Robert Braun-McGraw Hill.
  • A biologist Guide to principles and Techniques of Practical Biochemistry-K, Wilson and K.H. Goulding ElBS Edn.
  • Essentials of Biophysics, P Narayanan, New Age Int. Pub. New Delhi. 2000.
  •  Clark & Swizer. Experimental Biochemistry. Freeman, 2000.
  • Principles and Practice of Bioanalysis, R F Venn, Taylor and Francis, 2003.
  • Locquin and Langeron. Handbook of Microscopy. Butterwaths, 1983
  •  Boyer. Modern Experimental Biochemistry. Benjamin, 1993
  •  Freifelder. Physical Biochemistry. Freeman, 1982.
  • Wilson and Wlaker. Practical Biochemistry. Cambridge, 2000.
  • Cooper. The Cell-A Molecular Approach. ASM, 1997
  • John R.W. Masters. Animal Cell culture- A practical approach. IRL Press.
  •  Robert Braun. Introduction to instrumental analysis. McGraw Hill
  • David W. Mount's "Bioinformatics" [Cold Spring Harbor Press; ISBN 0879697121].
  • James Tisdall. Beginning Perl for Bioinformatics An Introduction to Perl for Biologists. Publisher: O'Reilly Media. October 2001.
  • Sharma, Munjal and Shankar. A text book of Bioinformatics. Rastogi Publications, Meerut, UP.
  • Essential Bioinformatics, Jin Xiong, John Wiley and Sons. 2006.


Academic Year: