BIOCHEMISTRY OF METABOLIC PROCESSES PRACTICAL

Paper Code: 
25CZOL412
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
This course will enable the students to -
1. Develop skills to differentiate between biomolecules based on various qualitative tests.
2. Learn analytical techniques to separate amino acids based on their physical properties.
3. Analyze the effect of temperature on the enzyme activity.
 
Course Outcomes: 

Course

Learning outcomes (at course level)

Learning and teaching strategies

Assessment Strategies

Course Code

Course Title

 

 

 

 

 

 

 

 

 

 

 

25CZOL-412

 

 

 

 

 

 

 

 

 

 

Biochemistry of metabolic processes Practical

CO:79 Perform qualitative tests for functional groups in biomolecules.Separate amino acid mixture by chromatography technique. CO:80 Investigate salivary amylase activity under optimal conditions.

CO:81 Explore pH and temperature effects on salivary amylase.

CO:82                      Demonstrate protein separation using SDS-PAGE

CO:83 Demonstrate skills and record the concepts in the form of practical files and reflect on personal learning experiences and experimental outcomes during viva voce.

CO:84                         Contribute effectively in course- specific interaction

The approach in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration,

Team teaching

Learning activities for the students: Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Chart preparation

Assessment Strategies Class             test, Semester end examinations

,           Quiz,

Solving problems                 in tutorials, Assignments, Presentation.

 

CONTENTS:
1. Qualitative tests of functional groups in carbohydrates, proteins and lipids.
2. Paper chromatography of amino acids.
3. Action of salivary amylase under optimum conditions.
4. Effect of pH and temperature on the action of salivary amylase.
5. Demonstration of protein separation by SDS-PAGE.
 
Essential Readings: 
● Cox, M.M and Nelson, D.L. (2008). Lehninger‘s Principles of Biochemistry, V Edition, W.H. Freeman and Co., New York.
● Berg, J.M., Tymoczko, J.L. and Stryer, L. (2007). Biochemistry, VI Edition, W.H. Freeman and Co., New York.
● Introductory practical Biochemistry, Sawhney S.K., Singh R. 2009. 7th edition, Narosa publishing house Pvt. Ltd. 
● Wilson,K. and Walker, J. Principles and techniques of practical biochemistry, Cambridge press.
 
SUGGESTED READINGS:
● Zubay G. Biochemistry, William C. Brown Pub.
● Standard methods of biochemical analysis. Thimmaiah, S.R. Kalyani Publishers, Noida
 
References: 
e-RESOURCES:
● Experimental Biochemistry- NPTEL- https://nptel.ac.in/courses/104105102
 
Academic Year: 
2025-2026
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