BIOCHEMISTRY OF METABOLIC PROCESSES PRACTICAL

Paper Code: 
24CZOL412
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
Course objectives: 
This course will enable the students to - 
1. Develop skills to differentiate between biomolecules based on various qualitative tests.
2. Learn analytical techniques to separate amino acids based on their physical properties.
3. Analyze the effect of temperature on the enzyme activity
 
Course Outcomes: 

Course

Learning outcomes

(at course level)

Learning and 

teaching strategies

Assessment

Strategies

 Course Code

Course Title

24CZOL-412

Biochemistry of  metabolic

processes

Practical

CO:79 Perform  qualitative tests for functional groups in biomolecules.Separate amino acid mixture by chromatography technique.

CO:80 Investigate salivary amylase activity under optimal conditions.

CO:81 Explore pH and temperature effects on salivary amylase.

CO:82 Demonstrate protein separation using SDS-PAGE

CO:83 Demonstrate skills and record the concepts in the form of  practical file and reflect on personal learning experiences and experimental outcomes during viva voce.

CO:84 Contribute effectively in course-specific interaction

The approach in  teaching: Interactive 

Lectures, Discussion, 

Tutorials, Reading  assignments, Demonstration,  Team teaching

 

Learning activities for the  students: Self-learning assignments, 

Effective questions, 

Simulation, Seminar 

presentation, Giving tasks,  Chart preparation

Assessment 

Strategies

Class test, Semester  end examinations,  Quiz, Solving  problems in tutorials,  Assignments, 

Presentation.

 

CONTENTS: 
1. Qualitative tests of functional groups in carbohydrates, proteins and lipids. 
2. Paper chromatography of amino acids. 
3. Action of salivary amylase under optimum conditions. 
4. Effect of pH and temperature on the action of salivary amylase. 
5. Demonstration of protein separation by SDS-PAGE. 
 

SCHEME FOR PRACTICAL EXAMINATION:

Scheme for CA  End Practical Examination

Exercise  1

Exercise II

File

Viva

Attendance

Total

 

5

 

 

5

 

10

 

5

 

5

 

30

 

Scheme for Semester End Practical Examination

Exercise I

Exercise II

Exercise III

Exercise IV

Spotting

File

Viva

Total

8

8

8

8

6x3=18

10

10

70

 

 

 

ESSENTIAL READINGS:  
● Cox, M.M and Nelson, D.L. (2008). Lehninger‘s Principles of Biochemistry, V Edition,  W.H. Freeman and Co., New York. 
● Berg, J.M., Tymoczko, J.L. and Stryer, L. (2007). Biochemistry, VI Edition, W.H.  Freeman and Co., New York. 
● Introductory practical Biochemistry, Sawhney S.K., Singh R. 2009. 7th edition, Narosa  publishing house Pvt. Ltd.
● Wilson,K. and Walker, J. Principles and techniques of practical biochemistry,  Cambridge press. 
SUGGESTED READINGS: 
● Zubay G. Biochemistry, William C. Brown Pub. 
● Standard methods of biochemical analysis. Thimmaiah, S.R. Kalyani Publishers, Noida 
 
e-RESOURCES: 
● Experimental Biochemistry- NPTEL- https://nptel.ac.in/courses/104105102 
 
Academic Year: 
2024-2025
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