BIOCHEMISTRY OF METABOLIC PROCESSES (PRACTICAL)

Paper Code: 
CZOL-412P
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

ourse 

Learning outcomes 

(at course level)

Learning and  teaching strategies

Assessment  Strategies 

Paper  

Code

Paper Title

CZOL-412P

BIOCHEMISTRY  OF METABOLIC PROCESSES

 

PRACTICAL

 

CO: Demonstrate various qualitative and quantitative biochemical techniques to differentiate between different biomolecules based on their physical and chemical properties.

CO:Analyze the effect of temperature on the enzymatic activity and gain hands-on expertise in the separation of various solutes using chromatographic techniques.

 

The approach in  teaching: 

Interactive Lectures,  Discussion, Tutorials,  Guest lectures,  PowerPoint  

presentations,  

Demonstration of  working of various  instruments 

Learning activities for  the students: 

Self learning  assignments, Effective  questions, Simulation,  Seminar presentation,  Giving tasks, 

● Class test 

● Semester  end  

examinatio 

ns, 

● Quiz,  

● Assignment s,  

● Presentatio n 

● Surprise  

test 

 

 

1. Qualitative tests of functional groups in carbohydrates, proteins and lipids.

2. Paper chromatography of amino acids.

3. Action of salivary amylase under optimum conditions.

4. Effect of pH and temperature on the action of salivary amylase.

5. Demonstration of protein separation by SDS-PAGE.

 
Essential Readings: 

ESSENTIAL READINGS: 

 

  • Cox, M.M and Nelson, D.L. (2008). Lehninger’s Principles of Biochemistry, V Edition, W.H. Freeman and Co., New York.

  • Berg, J.M., Tymoczko, J.L. and Stryer, L. (2007). Biochemistry, VI Edition, W.H. Freeman and Co., New York.

  • Introductory practical Biochemistry, Sawhney S.K., Singh R. 2009. 7th edition, Narosa publishing house Pvt. Ltd.

  •  Wilson,K. and Walker, J. Principles and techniques of practical biochemistry, Cambridge press.

.

SUGGESTED READINGS:

● Zubay G. Biochemistry, William C. Brown Pub.

● Standard methods of biochemical analysis. Thimmaiah, S.R. Kalyani Publishers, Noida

 

e-RESOURCES:

● Experimental Biochemistry- NPTEL- https://nptel.ac.in/courses/104105102

● Biochemistry virtual lab- Amrita- https://vlab.amrita.edu/?sub=3&brch=64

https://vlab.amrita.edu/?sub=3&brch=64&sim=1090&cnt=2

● Amrita Virtual Labs- http://biotech01.vlabs.ac.in/List%20of%20experiments.html:

 
Academic Year: