Biochemistry

Paper Code: 
ZOL 124
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

The course Objectives to provide students with a basic understanding of:

1.      Structure and functions of enzymes, protein , carbohydrates and lipids

2.      Metabolic reaction sand metabolic disorders

3.      Understand  the Bioenergetics of cell

 

Learning outcomes

By the end of the course, students should be able to:

1.      Describe the structure and functions of enzymes, protein , carbohydrates and lipids

2.      Describe the metabolic reaction sand metabolic disorders

Describe  the Bioenergetics of cell

10.00
Unit I: 
UNIT I

Biocatalysis

  • Classification and nomenclature of enzymes
  • Mechanism of enzyme action
  • Enzyme kinetics
  • Inhibition of enzyme
  • Isoenzymes and co-enzymes

Energy consideration

  • Biological oxidation and reduction
  • Fundamental reactions of biological oxidation

Redox potential and Electron transfer system enzymes 

14.00
Unit II: 
UNIT II

Carbohydrates

  • Definition , general properties, classification and configuration of carbohydrates

Metabolism

  • Catabolism of glucose with Stoichiometry, Glycolysis , Krebs cycle and Cori’s cycle
  • Phosphogluconate pathways(Pentose Phosphate Pathway)

 

Glycogenesis, Glycogenolysis and Gluconeogenesis

 

Inborn errors of metabolism: Galactosemia, Lactose Intolerance, Glycogen storage diseases.

 

 

12.00
Unit III: 
UNIT III

Lipids

  • Definition, general properties and classification
  • Structure and importance of -:
    1. Simple lipids- fats and wax
    2. Compound lipids- phospholipids, glycolipids, aminolipids and sulpholipids
    3. Steroids- cholesterol, bile acids, steroid hormones
    4. Lipoproteins
  • Lipid Metabolism- Beta oxidation and Lipogenesis.

 

  • Inborn errors of metabolism: Niemann pick disease, TaySach’sdisease,Gaucher’s disease, Krabbe’s disease

 

 

14.00
Unit IV: 
UNIT IV

General properties and classification of proteins

  • Amino acid structure and classification
  • Structure of protein-Primary, Secondary, Tertiary and Quartenary
  • Ramachandran plots
  • Globular and fibrous protein- motif in globular proteins

Protein sequencing

10.00
Unit V: 
UNIT V

Protein metabolism

  • Amino acid degradation:Deamination reaction- Oxidative and non oxidative deamination reaction, Transamination and Transdeamination, Decarboxylation, Ornithine cycle of urea formation , Fate of carbon skeleton of amino acids(Metabolism of individual amino acid excluded)

 

  • Inborn errors of metabolism:Alkaptonuria, Phenylketonuria, Maple Syrup, Tyrosinemia, Albinism ,Urea Disorders.

 

Essential Readings: 

Essential Readings:

  • Biochemistry, D.Voet and J.G.Voet, John Wiley & Sons, (2nd Edition), 1995.
  • Principles of Biochemistry, Lehninger ,Nelson and Cox, (3rd Edition), 1999
  • Principles and Techniques in Biochemistry and Molecular Biology, Edited by Keith Wilson and John Walker, Cambridge University Press, (6th Edition), 2007

 

References: 

Suggested Readings:

  • Outlines of Biochemistry; Conn, E.E. and Stump  P.K. ; John Wiley, New   York.
  • Principles of Biochemistry; Smith; McGraw hill, New York.
  • Biochemistry; Stryer, L; Freeman Publications.
  • Textbook of Medical Biochemistry; Ramakrishnan, S. Prasanan, K.G and Rajan, R.:  Orient Longman, Madras
  • Biochemistry and Molecular Biology by W.H. Elliot and Daphne C. Elliot, Oxford University Press, (3rd Edition), 2005.
  • Biochemistry, J. David Rawn, Panima Publishing Corporation, 1st Indian Reprint, 2004.
  • Physical Biochemistry, D Friefelder, W.H. Freeman & Company, 17th Reprint, 1999.
  • Understanding Chemistry, CNR Rao, University Press, Hyderabad 1999.
  • A Biologist's Guide to Principles and Techniques of Practical Biochemistry, K.Wilson& KH Goulding, ELBS Edition 1986.
  • Essentials of Molecular Biology, David Friefelder, Jones and Barllett Publications,7th Reprint, 2001.
  • Instant Notes- Biochemistry (1st Indian Edition), B.D. Hames and N.M. Hooper, Viva Books Pvt, Ltd., , 2001.    

 

Academic Year: 
2019-2020
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