Biochemistry

Paper Code: 
ZOL 124
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
Course objectives:
This course will enable the students to –
 
  • Enlighten the importance of biological macromolecules, structures and configurational properties. 
  • Examine the key role of biochemistry in the metabolic process. 
Course Outcomes (COs): 
 

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

ZOL 124

Biochemistry

The students will –

CO17: Analyse the concepts of biocatalysts,enzyme activity and its kinetics.

CO18: Acquire detailed knowledge about the structure, classification, physico-chemical properties and biological significance of carbohydrates, amino acids, proteins and lipids.

CO19: Illustrate the anabolic and catabolic pathways of different cellular biomolecules, rate-limiting steps and energetic pathways.

CO20: Correlate the defective enzymes and inborn errors of metabolism.

CO21: Assess the role of biomolecules in the metabolic processes.

Approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching

Learning activities for the students:

Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Field practical

Class test

Semester end examinations, Quiz,

Assignments,

Presentation

Individual and group projects

 

12.00
Unit I: 
UNIT I :
Biocatalysis
  • Classification and nomenclature of enzymes
  • Mechanism of enzyme action
  • Enzyme Kinetics
  • Inhibition of enzyme
  • Isoenzymes and coenzymes
  • Energy consideration
 
Biological oxidation and reduction
  • Fundamental reactions of biological oxidation
  • Redox potential and Electron transfer system enzymes
14.00
Unit II: 
UNIT II :
Carbohydrates
 
  • Definition, general properties, classification and configuration of carbohydrates
 
Metabolism
 
  • Catabolism of glucose with Stoichiometry, Glycolysis, Krebs cycle and Cori’s cycle
  • Phosphogluconate pathways(Pentose Phosphate Pathway) Glycogenesis, Glycogenolysis and Gluconeogenesis
 
Inborn errors of metabolism:
 
  • Galactosemia, Lactose Intolerance, Glycogen storage diseases.
 
 
 
 
 
 
 
12.00
Unit III: 
UNIT III
Lipids
 
  • Definition, general properties and classification

Structure and importance of-:

  • Simple lipids- fats and wax
  • Compound lipids- phospholipids, glycolipids, amino lipids and sulpholipids
  • Steroids- cholesterol, bile acids, steroid hormones
  • Lipoproteins
Lipid Metabolism
 
  • Beta oxidation and Lipogenesis.
  • Inborn errors of metabolism: Niemann pick disease, Tay Sach’s disease, Gaucher’s disease, Krabbe’s disease
 
14.00
Unit IV: 
UNIT IV
General properties and classification of proteins
 
  • Amino acid structure and classification
  • Structure of protein-Primary, Secondary, Tertiary And Quaternary
  • Ramachandran plots
  • Globular and fibrous protein- motif in globular proteins
  • Protein Sequencing
 
10.00
Unit V: 
UNIT V
Protein metabolism
 
  • Amino acid degradation: Deamination reaction- Oxidative and non oxidative deamination reaction, Transamination and Trans deamination, Decarboxylation, Ornithine cycle of urea formation , Fate of carbon skeleton of amino acids(Metabolism of individual amino acid excluded)
  • Inborn errors of metabolism: Alkaptonuria, Phenylketonuria, Maple Syrup, Tyrosinemia, Albinism ,Urea Disorders
 
Essential Readings: 
  • Biochemistry, D.Voet and J.G.Voet, John Wiley & Sons, (2nd Edition),1995.
  • Principles of Biochemistry, Lehninger ,Nelson and Cox, (3rd Edition),1999.
  • Biochemisty, U. Satyanarayan and U. Chakrapani (4thedition)
  • Text Book of Medical Physiology; Chatterjee, M.N and Shinde, R.; Jaypeebrothers.
  • Outlines of Biochemistry; Conn, E.E. and Stumpf P.K. ; John Wiley, NewYork.
  • Principles of Biochemistry; Smith; McGraw hill, NewYork.
  • Biochemistry; Styrer, L; FreemanPublications.
  • Textbook of Medical Biochemistry; Rama krishnan, S. Prasanan, K.G and Rajan,R.: Orient Longman,Madras
  • Principles and Techniques in Biochemistry and Molecular Biology, Edited byKeith Wilson and John Walker, Cambridge University Press, (6th Edition),2007.
  • Biochemistry and Molecular Biology by W.H. Elliot and Daphne C. Elliot,Oxford University Press, (3rd Edition),2005.
  • Biochemistry, J. David Rawn, Panima Publishing Corporation, 1st Indian Reprint, 2004.
  • Understanding Chemistry, CNR Rao, University Press, Hyderabad1999.
  • Essentials of Molecular Biology, David Friefelder, Jones and Barllett Publications,7th Reprint,2001.
  • Instant Notes- Biochemistry (1st Indian Edition), B.D. Hames and N.M.Hooper, Viva Books Pvt, Ltd.,2001.
 
Academic Year: 
2021-2022
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