BASICS OF MOLECULAR BIOLOGY (THEORY)

Paper Code: 
25CZOL513
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
This course will enable the students to –
1. Develop an understanding of the life processes at the subcellular and molecular levels.
2. Differentiate and evaluate important aspects of DNA and molecular genetics including gene cloning, sequencing and gene mapping in addition to the powerful techniques that revolutionized the pharmaceutical, health and agricultural industries.
 
Course Outcomes: 

Course

Learning outcomes (at course level)

Learning and teaching strategies

Assessmen t Strategies

Course Code

Course Title

 

 

 

 

 

 

 

 

 

 

 

 

 

 

25CZOL 513

 

 

 

 

 

 

 

 

 

 

 

 

Basics of Molecular Biology THEORY

The students will:

CO:109 Understand replication mechanism in prokaryotes and eukaryotes

CO:110 Appraise the structure and types of Nucleic acids, their characteristic features and occurrence.

CO:111    Develop              detailed knowledge          about     the process and importance           of DNA replication in both prokaryotes and eukaryotes and              protein                machinery involved in it.

CO:112                              Analyse comprehensive knowledge     about     the process of Transcription. CO:113      Develop    an understanding of translation along with the mechanisms   of                   post transcriptional and post translational processes in the cell

CO:114 Contribute effectively in course-specific interaction.

The approach in teaching: Interactive Lectures, Discussion, Tutorials,

Reading assignments, Demonstration, Guest lectures

 

Learning activities for the students: Self learning, assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Regular activities like quiz, surprise test, explanation of given task

Class test Semester end examinati ons, Quiz,

Assignment, Presentation Surprise test Open book test

 

12.00
Unit I: 
Nucleic Acids
Salient features of DNA and RNA Watson and Crick model of DNA
Semi-conservative, bidirectional and semi-discontinuous replication, DNA Replication: DNA Replication in prokaryotes and eukaryotes, Mechanism of DNA replication 
 
12.00
Unit II: 
Transcription
RNA polymerase and transcription unit,
Mechanism of transcription in prokaryotes and eukaryotes Transcription factors
 
12.00
Unit III: 
Translation
Genetic code, Degeneracy of the genetic code and Wobble Hypothesis Process of protein synthesis in prokaryotes:
Ribosome structure and assembly in prokaryotes, fidelity of protein synthesis, aminoacyl tRNA synthetases and charging of tRNA;
Proteins involved in initiation, elongation and termination of polypeptide chain
 
12.00
Unit IV: 
Post Transcriptional Modifications and Processing of Eukaryotic RNA
Capping
Polyadenylation
Concept of introns and exons
Splicing mechanism, alternative splicing
 
12.00
Unit V: 
Transcription regulation
Gene Regulation: Transcription regulation in prokaryotes: Principles of transcriptional regulation with examples from lac operon and trp operon
Transcription regulation in eukaryotes: Activators, repressors, enhancers, silencer elements; Gene silencing
 
Essential Readings: 
● Cell Biology by C.B. Powar; Himalaya Publishing House.
● Cell and Molecular Biology; Phillip Sheeler Donald E. Bianchi; John Wiley and Sons.
 
References: 
● The Cell: A Molecular Approach. Cooper, G. Houseman G.R. SinaeurAssoc, New York
● Becker, W.M., Kleinsmith, L.J., Hardin. J. and Bertoni, G. P. (2009). The World of the Cell.VII Edition. Pearson Benjamin Cummings Publishing, San
● Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts,
● Peter Walter: Molecular Biology of the Cell, IV Edition.
● Cooper G. M. and Robert E. Hausman R. E. The Cell: A Molecular Approach, V Edition, ASM Press and Sinauer Associates.
● De Robertis, E.D.P. and De Robertis, E.M.F. (2006).Cell and Molecular Biology.VIII Edition. Lippincott Williams and Wilkins, Philadelphia.
● Karp, G. (2010) Cell and Molecular Biology: Concepts and Experiments. VI Edition.John Wiley and Sons.Inc.
● Lewin B. (2008). Gene XI, Jones and Bartlett
● McLennan A., Bates A., Turner, P. and White M. (2015). Molecular Biology IV Edition.GS, Taylor and Francis Group, New York and London.
● Molecular Cell biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore, Scientific American Books,USA,1994.
● Cell & Molecular Biology, (8th edition), E D P Roberties & E M F Roberties, Lippincott Williams & Wilkins, 2005
e-RESOURCES:
JOURNALS:
• The Journal of Cell and Molecular Biology
• Cellular and Molecular Biology
• Cellular and Molecular Life Sciences 
 
Academic Year: 
2025-2026
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